161,256 results

1. pET28a-EGFP-CNA35

   Plasmid

   #61603

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with EGFP

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   EGFP and CNA35
   

   Use

   Tags

   His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF3549

   Plasmid

   #145025

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-pegRNA-puro backbone

   Plasmid

   #214085

   Purpose

   Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UAS:mCherry

   Plasmid

   #170994

   Purpose

   UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   UAS:mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic 14x UAS:Gal4

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sfGFP-C1

   Plasmid

   #54579

   Purpose

   Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Michael Davidson, Geoffrey Waldo

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-JAK1

    Plasmid

    #174572

    Purpose

    Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    JAK1 (JAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LDB-3-p65

    Plasmid

    #158984

    Purpose

    Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-3-p65
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHDM-VSV-G

    Plasmid

    #164440

    Purpose

    Encodes VSV-G protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENN236_CRISPRcharm_Kv2

    Plasmid

    #220839

    Purpose

    CRISPRcharm Kv2 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv2
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-M2rtTA

    Plasmid

    #20342

    Purpose

    Lentiviral plasmid expressing the reverse tetracycline transactivator

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

    Insert

    M2rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmScarlet-I3_C1

    Plasmid

    #189756

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IDP456

    Plasmid

    #87002

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    barcode landing site-mCherry-4xboxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti EF1a GFP-mCherry-mPlin1

    Plasmid

    #189005

    Purpose

    Fluorescent reportor for lipophagy

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    GFP, mChery, Plin1 (Plin1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pHDM-Hgpm2

    Plasmid

    #164441

    Purpose

    Encodes codon-optimized HIV gagpol for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    Codon Optimized HIV-1 GagPol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV4-p52

    Plasmid

    #23289

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IDP432

    Plasmid

    #87000

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Insert

    SNAP-Nlg1AB-1xλN(i)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCF159_BRL

    Plasmid

    #225962

    Purpose

    CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-BRL (env protein)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC18R6KT-mini-Tn7T-Km

    Plasmid

    #64969

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pLVX_DSB_Spectrum-V1

    Plasmid

    #192474

    Purpose

    Lentiviral DSB Spectrum V1 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid

    #105530

    Purpose

    AAV expression of EGFP from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCas9_GFP

    Plasmid

    #44719

    Purpose

    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Tobias Meyer, Sabrina Spencer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hSyn-mCherry

    Plasmid

    #114472

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5_mCherry_P2AT2A_GFP-nb

    Plasmid

    #238232

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5_mCherry-P300

    Plasmid

    #238258

    Purpose

    For overexpression of mCherry-P300

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5-Flag-BRD4-WT

    Plasmid

    #90331

    Purpose

    expresses Wild type BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUC19

    Plasmid

    #50005

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDisCoTune

    Plasmid

    #173713

    Purpose

    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

    Depositor

    Morten Norholm

    Article

    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

    Inserts

    ERV1 (ERV1 Budding Yeast)
    PDI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; crRNA under control of PermE*

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLV-mitoGFP

    Plasmid

    #44385

    Depositor

    Pantelis Tsoulfas

    Article

    Kitay et al Hum Mol Genet. 2013 Jan 24.

    Insert

    mitoGFP (COX8A Human)
    

    Use

    Lentiviral

    Tags

    Cox8 targeting sequence and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSM-mStayGold

    Plasmid

    #234317

    Purpose

    codon-optimized mStayGold with synthetic introns for the expression in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

    Insert

    C. elegans codon optimized mStayGOld
    

    Use

    Tags

    mStayGold

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti6.3/V5-DEST-GFP

    Plasmid

    #40125

    Depositor

    Lynda Chin

    Article

    Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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48. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. GFP-rab11 WT

    Plasmid

    #12674

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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