165,343 results

1. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMPP-mCherry-hTRIM21

   Plasmid

   #104972

   Purpose

   Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   SFFV

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-TDP-43

   Plasmid

   #235487

   Purpose

   Expression of human TDP-43-EGFP

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. DJ-1 pET15b

   Plasmid

   #60678

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

   Insert

   DJ-1 (PARK7 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. WT GBA pcDNA3.1

   Plasmid

   #188580

   Purpose

   Ectopic expression of wild-type GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytoplasmic PancACe

   Plasmid

   #215708

   Purpose

   Expresses acetyl-CoA biosensor in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

   Plasmid

   #236368

   Purpose

   Overexpression of HyperdLbCas12a in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   HyperdLbCas12a
   

   Use

   CRISPR and Lentiviral

   Mutation

   D156R, D235R, E292R, D350R

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRNA-CleanCap entry vector

   Plasmid

   #188249

   Purpose

   Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-TDP-43 (Q331K)

    Plasmid

    #235488

    Purpose

    Expression of human TDP-43-EGFP with human ALS-causing Q331K mutation

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q331K

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-KRAS-G12D

    Plasmid

    #116423

    Purpose

    Lentiviral expression of KRAS G12D

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Mutation

    G12D

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLTR-RD114A

    Plasmid

    #17576

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    RD114 envelope glycoprotein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA6 asyn WT

    Plasmid

    #107425

    Purpose

    Mammalian expression of human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein (SNCA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIH-TFAM-GFP

    Plasmid

    #113704

    Purpose

    Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TFAM (Tfam Mouse)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKP332 (Lenti-OSK1)

    Plasmid

    #21627

    Depositor

    Tim Townes

    Article

    Chang et al Stem Cells. 2009 May . 27(5):1042-9.

    Insert

    hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. eGFP-ova-C1

    Plasmid

    #163524

    Purpose

    Expresses both eGFP and chicken ovalbumin in mammalian cells

    Depositor

    Thomas Weber

    Insert

    enhanced-GFP-chicken ovalbumin tandem
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti6-DEST PINK1-V5 WT

    Plasmid

    #13320

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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25. pG1K

    Plasmid

    #71741

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Bacterial

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. TMEM106B-EGFP

    Plasmid

    #210366

    Purpose

    Expresses TMEM106B fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TMEM106B (TMEM106B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SPLICS LY-MT Short P2A

    Plasmid

    #213614

    Purpose

    Detect the short-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Expression

    Mammalian

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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31. hAAVS1 1L TALEN

    Plasmid

    #35431

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1L TALEN
    

    Expression

    Mammalian

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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32. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Expression

    Mammalian

    Mutation

    A206K

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

34. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDonor_Tn[himar1C9]

    Plasmid

    #213906

    Purpose

    Mariner transposon with ATc-inducible himar1C9 transposase

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCI-neo-sOVA

    Plasmid

    #25098

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    soluble ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYFP-RPB1aAmr

    Plasmid

    #75284

    Purpose

    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

    Depositor

    Robert Singer, Xavier Darzacq

    Article

    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    N792D alpha-amanitin–resistant

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge