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Plasmid#187027PurposeExpresses IDO2 and GFP (seperately) in mammalian cellsDepositorInsertIndoleamine 2,3-dioxygenase 2 (IDO2 Human)
UseTagsP2A-GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kv-ArcLight-ST
Plasmid#182319PurposeSoma-targeted fluorescent voltage indicator with left-shifted voltage-dependencyDepositorInsertKv-ArcLight-ST
UseTagsExpressionMammalianMutationMutation in an amino acid Q217E of ArcLight-MTPromoterCAGAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]
Plasmid#159796PurposeHistamine based negative selection marker to paralyze animals with arraysDepositorInsertpEXP[Prpl-3 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV2 Dvl-2-Myc
Plasmid#42194DepositorInsertDvl 2 (Dvl2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0208
Plasmid#142556PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP2 (FOXP2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 tTA SDHB
Plasmid#177977PurposeAll-in-one dox-repressible expression of cDNADepositorInsertSDHB (SDHB Human)
UseLentiviralTagsExpressionMammalianMutationSilent point mutations to SDHB sgRNA targetPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_2XMTS-MicroID2-FLAG
Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTripz NEO DEST
Plasmid#209072PurposeModified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interestDepositorTypeEmpty backboneUseLentiviral; Gateway destination vector to be used…Tags3X FLAGExpressionMutationPromoterCMVAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-TurboID-HA
Plasmid#219810PurposeSecreted HA-tagged TurboID for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
UseTagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1906
Plasmid#141883PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF7L2 (TCF7L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX SgK269 WT
Plasmid#68028Purposeexpression of Sgk269 in mammalian cellsDepositorInsertPEAK1 (PEAK1 Human)
UseRetroviralTagsFLAG/myc, HA, and IRES-DsRedExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS-Flag-BRAFV600E
Plasmid#126615PurposeRetroviral expression of Flag tagged human oncogenic BRAFV600EDepositorInsertBRAF (BRAF Human)
UseRetroviralTagsFlagExpressionMammalianMutationV600EPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)
Plasmid#208166PurposeAiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytesInsertiCreR297T
UseAAVTagsExpressionMutationNAPromoterBeta Globin minimal promoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE
Plasmid#111393PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N BNLF2a
Plasmid#37941DepositorInsertBNLF2a (BNLF2a H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NEGR1-Fc(DAPA)-AviTag-6xHis
Plasmid#156728PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNEGR1 (NEGR1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQH
Plasmid#48096PurposeCumate-inducible expression of HA-tagged fusion proteinDepositorTypeEmpty backboneUseTags3xFLAG and HAExpressionBacterialMutationPromoterPQ5Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_degron-60A
Plasmid#200396PurposeA plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase - degron
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0723
Plasmid#142226PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKB1 (NFKB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSgk1_v2 Flag
Plasmid#86040Purposefull length human Sgk1_i2 with Flag tagDepositorInsertAlternate transcript v2 of human Sgk1 (SGK1 Human)
UseTagsFLAGExpressionMammalianMutationv2PromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[AAV]
Plasmid#40168DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF2095
Plasmid#141918PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertST18 (ST18 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-V2_mMSH2
Plasmid#186156PurposesgRNA targeting murine Msh2 geneDepositorInsertMsh2_gRNA (Msh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GW1-mScarlet-Epac-GeNL
Plasmid#207874PurposeMammalian expression of the FRET/BRET cAMP sensorDepositorInsertmScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL1-E2C
Plasmid#180421PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.DepositorInsertE2 Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRG215
Plasmid#64525PurposeCentromeric E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6633
Plasmid#106162PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1207
Plasmid#144089PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCASZ1 (CASZ1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_7 [pSa ORI]
Plasmid#198923PurposeLevel 0 partDepositorInsertpSa ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBUN250
Plasmid#60672Purposeoverexpression of M. smegmatis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTagsExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xHA-mASL
Plasmid#34577PurposeNOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.DepositorInsertASL (Asl Mouse)
UseTags1xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
gB gag LV
Plasmid#12328DepositorInsertgag/pol
UseLentiviralTagsExpressionMammalianMutationThe CypA binding region of the WT gag was replace…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
Wnt7A STOP
Plasmid#35877DepositorInsertWnt7A (WNT7A Human)
UseEntry vectorTagsExpressionMutationNonePromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-TDP-43 (Q331K)
Plasmid#235488PurposeExpression of human TDP-43-EGFP with human ALS-causing Q331K mutationDepositorInsertTARDBP (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationQ331KPromoterAvailable sinceApril 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-DD-T6B-WT-YFP
Plasmid#235143PurposeExpresses the inducible T6B peptide fused with DHFR and YFP under the human synapsin promoterDepositorInsertDHFR-fused T6B peptide
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLAG-HA-T6B-MUT-YFP
Plasmid#235140PurposeExpresses the mutant T6B peptide fused with YFP under the human synapsin promoterDepositorInsertmutant T6B peptide
UseAAVTagsFLAG/HAExpressionMutation5 tryptophan residues of T6B-WT mutatedPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLAG-HA-T6B-WT-YFP
Plasmid#235139PurposeExpresses the T6B peptide fused with YFP under the human synapsin promoterDepositorInsertT6B peptide
UseAAVTagsFLAG/HAExpressionMutationPromoterhSynAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
UseTagsExpressionMutationPromoterp14GAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
UseTagsExpressionBacterialMutationPromoterPEM7 and unknownAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorInsertHIS-HA-CMYC-p53 (TP53 Human)
UseTagsHIS-HA-CMYCExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-His-VTCN1
Plasmid#232664Purposetransient expresses VTCN1 in mammalian cellsDepositorInsertVTCN1 (VTCN1 Human)
UseTagsHis and His (retained after signal peptide cleava…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneUseTagsHis6 and Twin-Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only