167,150 results
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Plasmid#1791PurposeMammalian expression of flag-tagged SIRT1DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only
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pJRH-1365 LV EF1a-CD28 IRES-EGFP
Plasmid#201921Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD28DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.tgoGAP1
Plasmid#78737PurposeExpression of Ca2+ sensor GAP1 in the trans Golgi apparatusDepositorInserttgoGAP1
TagsN terminal 81 amino acids of human galactosyltran…ExpressionMammalianMutationD117A/D119A/D163A in aequorin moietyPromoterCMVAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-84 mRFP
Plasmid#206926PurposeThe micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.DepositorInsertmRFP
UseAAVExpressionMammalianPromoterMP-84Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)PromoterEF1aAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ:RUBY
Plasmid#160909PurposeRUBY under the control of Ubiquitin promoter/marker for transformationDepositorInsertRUBY and UBIQUITIN promoter
ExpressionPlantPromoterUBIQUITINAvailable SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A
Plasmid#169826PurposeExpresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationT456A S1406A S1859A; TCCC -> AGTC silent mutat…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fos-FlpOERT2
Plasmid#241239PurposeExpression of FlpOERT2 under the Fos promotor in mammalian cellsDepositorInsertFos-FlpoERT2
UseAAVAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
Tags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFEndoNA
Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK435
Plasmid#236573PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_BDepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-H2A-10
Plasmid#55054PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
perbB2-EGFP
Plasmid#39321DepositorAvailable SinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV
Plasmid#216156PurposeExpresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
ExpressionBacterialPromoternative phage promotersAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 Stop
Plasmid#84895PurposeDONOR vector for Gateway cloning of TAF15DepositorAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralExpressionMammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
ExpressionMammalianAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12C
Plasmid#58901PurposeExpresses human Kras with a G12C mutationDepositorAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN(deltaNLS)-pmGFP
Plasmid#26867PurposeExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membraneDepositorInsertCIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
TagsEGFPExpressionMammalianMutationNLS sequences mutatedPromoterCMVAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
TagsmNeonGreen2 on ORF1pExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV8, and AAV9InserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
TagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA32 (MSW#497)
Plasmid#208068PurposeExpression of GFP-tagged hsRPA2 in human cellsDepositorAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA14 (MSW#503)
Plasmid#208069PurposeExpression of GFP-tagged hsRPA3 in human cellsDepositorAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-TM
Plasmid#207320PurposeAllows cloning of protein coding sequences to be incorportated into envelope of virionsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO MYC3
Plasmid#177848PurposeBacterial Expression of MYC3DepositorAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_miRFP670
Plasmid#113726PurposeExpresses the fluorescent protein miRFP670 in a lentiviral backboneDepositorInsertmiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SMC3
Plasmid#222554PurposePiggyBac transposon plasmid for doxycycline inducible expression of SMC3DepositorInsertSMC3 (SMC3 Human)
ExpressionMammalianAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWRS3001_VCR1_WCR3
Plasmid#192206PurposeVCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backboneDepositorInsertVCR1-WCR3 tracrRNA
ExpressionMammalianAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfGFPBb
Plasmid#118231PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFPDepositorInsertflagellin promoter driven msfGFP
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mNG2(11)_Clathrin light chain
Plasmid#82608PurposeExpresses mNG2(11) tagged clathrin light chain in mammalian cellsDepositorInsertmNG2(11)_Clathrin light chain
ExpressionMammalianAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYU-2-Env
Plasmid#133997PurposeEnv gene from HIV-1 YU-2 in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI flag PCAF
Plasmid#8941DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic cpGFP
Plasmid#215709PurposeExpresses cpGFP in the cytoplasmDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD-CTwinStrep
Plasmid#45939Purposeuse to create C-terminal Twin-Strep-tag fusion proteinsDepositorTypeEmpty backboneTagsTwin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
hNTo2-qgRNA-pYJA5
Plasmid#217782PurposeNon-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloningDepositorInsertQuadruple sgRNA-expression cassette for nontargeting control for gene ablation
UseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterhuman U6, mouse U6, human H1, human 7SKAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
APEX2-NLS
Plasmid#124617Purposeproximity biotinylationDepositorInsertAPEX2-NLS
ExpressionMammalianPromoterCMVAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
TagsV5ExpressionMammalianPromoterTRE3GAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTagBFP2
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorInsertmTagBFP2
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only