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177,145 results

Showing: 2201 - 2250 of 177145 results

  1. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    139998-plasmid-map-sequence-id-394975

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  2. pAAV.CMV.Luc.IRES.EGFP.SV40

    Plasmid
    #105533
    Purpose
    AAV expression of Luciferase and EGFP from CMV promoter
    Depositor
    James M. Wilson
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    105533-plasmid-map-sequence-id-202367

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  9. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid
    #50477
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85 Articles
    50477-plasmid-map-sequence-id-370683

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  10. pcDNA3.1(+) Drp1

    Plasmid
    #34706
    Depositor
    David Chan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Drp1 (Dnm1l Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    34706-plasmid-map-sequence-id-227765

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  12. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid
    #28289
    Purpose
    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)
    Depositor
    Alex Chenchik, Gus Frangou
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available since
    April 20, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    28289-plasmid-map-sequence-id-197795

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  21. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Publication
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    99866-plasmid-map-sequence-id-192399

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  22. pMA4008

    Plasmid
    #70109
    Purpose
    mtDNA destruction/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Publication
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP
    Herpes simplex virus UL12.5M185
    Tags
    none
    Expression
    Mammalian
    Mutation
    N-terminal deletion of UL12.5
    Promoter
    CMV and RSV
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    70109-plasmid-map-sequence-id-364432

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  32. pCMV-P35

    Plasmid
    #1347
    Depositor
    Li-Huei Tsai
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P35 (CDK5R1 Human)
    Expression
    Mammalian
    Available since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    1347-plasmid-map-sequence-id-127179

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  35. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    MMLV Reverse Transcriptase
    Tags
    10x His
    Expression
    Bacterial
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

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  36. pET-28a(+)-FGF2-G3

    Plasmid
    #135521
    Purpose
    Engineered form of fibroblast growth factor 2 with improved thermostability
    Depositor
    Paul Burridge
    Publication
    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221
    Insert
    fibroblast growth factor 2 (FGF2 Human)
    Tags
    6x His tag and Agg Thrombin Tag
    Expression
    Bacterial
    Mutation
    Codon optimized for E. Coli production
    Promoter
    T7 Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135521-plasmid-map-sequence-id-273044

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  40. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Publication
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101139-plasmid-map-sequence-id-194900

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  42. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Publication
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    61628-plasmid-map-sequence-id-396550

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  43. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    60226-plasmid-map-sequence-id-176980

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  45. pmEGFP-C1

    Plasmid
    #36412
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    36412-plasmid-map-sequence-id-438461

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Showing: 2201 - 2250 of 177145 results