19,162 results

1. pN1-GCaMP6m-XC

   Plasmid

   #111543

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

   Depositor

   Xiaodong Liu

   Article

   Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

   Insert

   GCaMP6m-XC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. phage UbiC G3BP1-GFP-GFP

   Plasmid

   #119950

   Purpose

   Lentiviral vector expressing G3BP1-GFP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo RYR-1

   Plasmid

   #75113

   Purpose

   Clone encoding rabbit ryanodine receptor in mammalian expression vector

   Depositor

   Paul Allen, Robert Dirksen

   Article

   Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

   Insert

   Ryanodine receptor 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC18T-mini-Tn7T-Gm-eyfp

   Plasmid

   #65031

   Purpose

   GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBsaBM3

   Plasmid

   #85102

   Purpose

   P450 BM3 variant (A74G/C773S/C810S)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450 BM3
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   A74G/C773S/C810S

   Promoter

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-GFAP-hM4D(Gi)-mCherry

   Plasmid

   #50479

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

   Depositor

   Bryan Roth

   Has Service

   AAV5

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-mRuby2

    Plasmid

    #40260

    Purpose

    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    mRuby2
    

    Use

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Pveg

    Plasmid

    #55173

    Purpose

    Very strong constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    veg promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    veg

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pm-iATPSnFR1.1

    Plasmid

    #102549

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRN11

    Plasmid

    #84455

    Purpose

    shuttle plasmid for sarA P1-mCherry expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mCherry
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCR4-24XMS2SL-stable

    Plasmid

    #31865

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    24xMS2 Stem Loops - Stable
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pActP-GCaMP6m

    Plasmid

    #64714

    Purpose

    visualizing calcium in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    GCaMP6m
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4-FLAG-Jhdm2a-H1122A

    Plasmid

    #38140

    Depositor

    Toshinobu Nakamura, Toru Nakano

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a-H1122A (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115; H1122A

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-mBeRFP

    Plasmid

    #175173

    Purpose

    large-stokes shift red fluorescent protein mBeRFP

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    mBeRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-Raichu-RhoA-CR

    Plasmid

    #40258

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Raichu-RhoA-CR (RHOA Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p2CT-His-MBP-Lsh_C2c2_WT

    Plasmid

    #83487

    Purpose

    Bacterial expression of WT L. shahii C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lsh_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57-MCS1-2A-MCS2

    Plasmid

    #71782

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Mm.cargo(Cas9)

    Plasmid

    #174865

    Purpose

    Expresses mouse Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by MmPeg10 UTRs
    

    Use

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-GCaMP6s

    Plasmid

    #171156

    Purpose

    Encodes GCaMP6s

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PUC57_BAG3wt-eGFP

    Plasmid

    #98182

    Purpose

    Codes for full length, GFP tagged, human wildtype Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3wt-eGFP (BAG3 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUltra-Plus

    Plasmid

    #161775

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Type

    Empty backbone

    Use

    Lentiviral; Multicistronic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR223-IRAK4

    Plasmid

    #23749

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    IRAK4 (IRAK4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pBV-Luc wt MBS1-4

    Plasmid

    #16564

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

    Insert

    c-MYC binding sites (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti 4.1 Ex miR200c-141

    Plasmid

    #35534

    Purpose

    3rd generation lentiviral vector

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    miR200c-141 genomic cluster (MIR200C, MIR141 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-VSVG

    Plasmid

    #64084

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    myc, His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLAG-SENP8

    Plasmid

    #18066

    Depositor

    Edward Yeh

    Article

    Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

    Insert

    SENP8 (SENP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pLKO.1 puro shRNA beta-catenin

    Plasmid

    #18803

    Purpose

    3rd gen lentiviral vector for knocking down beta-catenin gene expression

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    b-catenin shRNA (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pFBDM

    Plasmid

    #110738

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBabe puro c-met WT

    Plasmid

    #17493

    Depositor

    Joan Brugge

    Article

    Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

    Insert

    c-Met (Met Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GFP-Rab5B

    Plasmid

    #61802

    Purpose

    Human Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    RAB5B (RAB5B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-Egr1

    Plasmid

    #11729

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    Egr-1 (Egr1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHAGE-PDGFRA

    Plasmid

    #116769

    Purpose

    Lentiviral expression of PDGFRA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. VPS29-YFP

    Plasmid

    #174520

    Purpose

    Expresses human VPS29 with a C-terminal YFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50454

    Purpose

    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLJM1-Empty

    Plasmid

    #91980

    Purpose

    Empty backbone

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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