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4,203 results

Showing: 3051 - 3100 of 4203 results

  4. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  13. pcDNA3-TLR5-CFP

    Plasmid
    #13019
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 5 (TLR5 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13019-plasmid-map-sequence-id-490371

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  22. pLEX_306

    Plasmid
    #41391
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41391-plasmid-map-sequence-id-473523

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  25. pCEP4-myc-ACE2

    Plasmid
    #141185
    Purpose
    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141185-plasmid-map-sequence-id-274835

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  27. pDONR207 SARS-CoV-2 NSP3

    Plasmid
    #141257
    Purpose
    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141257-plasmid-map-sequence-id-288044

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  36. pPD95_75

    Plasmid
    #1494
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1494-plasmid-map-sequence-id-372920

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  43. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  44. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49436-plasmid-map-sequence-id-251833

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  45. pCALNL-GFP

    Plasmid
    #13770
    Purpose
    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    EGFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13770-plasmid-map-sequence-id-7759

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  47. pTiger

    Plasmid
    #1728
    Purpose
    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system
    Depositor
    Garry Nolan
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Aug. 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    1728-plasmid-map-sequence-id-105963

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  50. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid
    #50479
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50479-plasmid-map-sequence-id-166303

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Showing: 3051 - 3100 of 4203 results