159,238 results

1. pRG205MX

   Plasmid

   #64535

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRG206MX

   Plasmid

   #64536

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMAL-c4X

   Plasmid

   #75288

   Purpose

   Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 19, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBiFC-PBZ-VN

   Plasmid

   #110646

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus N-terminus

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   DDK and VN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-PBZ-VC

   Plasmid

   #110648

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus C-terminus

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   HA and VC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPS22 (pET23a-Cas10/Csm/csm2H6N)

   Plasmid

   #70038

   Purpose

   Bacterial expression of the S. epidermidis Cas10·Csm complex

   Depositor

   Luciano Marraffini

   Article

   Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

   Insert

   Cas10/Csm complex
   

   Use

   Tags

   6xHIS on N terminus of Csm2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-PUM1

   Plasmid

   #120384

   Purpose

   Bacterial expression of MBP-Pumillio1 fusion protein

   Depositor

   Igor Ulitsky

   Article

   Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

   Insert

   PUM1 (PUM1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAC_N_ELF2

   Plasmid

   #187737

   Purpose

   MAC-tagged gene expression of human ELF2

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   ELF2 (ELF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPet-His

    Plasmid

    #14031

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    YPet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PIK3CA - Exon9 E545K

    Plasmid

    #16642

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E545K; see note below

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

    Plasmid

    #176541

    Purpose

    human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    OgeuIscB omegaRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid

    #170255

    Purpose

    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    SV40 Large T Antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSKBAD2

    Plasmid

    #15335

    Depositor

    Hideo Iwai

    Article

    Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

    Insert

    DnaE C-intein
    

    Use

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LiOn-CAG∞GFP

    Plasmid

    #154015

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. ER-GCaMP6-150

    Plasmid

    #86918

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-150
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF3505

    Plasmid

    #144981

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF12 (KLF12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-PE7-SB

    Plasmid

    #228869

    Purpose

    PE7 connected to MLH1-SB via 2A sequence

    Depositor

    Sangsu Bae

    Insert

    PE7-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5-PLA2G15-6xHis WT

    Plasmid

    #213603

    Purpose

    Expresses PLA2G15-6xHis in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    Phospholipase A2 Group XV (PLA2G15 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUGI-NLS

    Plasmid

    #101091

    Purpose

    Expresses UGI in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIVM_26

    Plasmid

    #204501

    Purpose

    Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    0

    Promoter

    AmpR

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF3237

    Plasmid

    #144713

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

    Plasmid

    #170318

    Purpose

    Expresses FLAG and EGFP tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-FOXP2

    Plasmid

    #153146

    Purpose

    Expression of Flag tagged human FOXP2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP2 (FOXP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCM62

    Plasmid

    #45826

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Broad host range cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HPV16 E7 TCR

    Plasmid

    #122728

    Purpose

    Express HPV16 E7 TCR

    Depositor

    Christian Hinrichs

    Article

    Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

    Insert

    HPV16 E7 TCR
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TFORF3233

    Plasmid

    #144709

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    JUNB (JUNB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti6/V5-p53_R175H

    Plasmid

    #22936

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R175H Mutant

    Promoter

    Available since

    March 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-ccdB-3xFLAG

    Plasmid

    #214984

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TFORF2708

    Plasmid

    #144217

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARX (ARX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid

    #197511

    Purpose

    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and BPNLS-NpuC(intein)

    Expression

    Mutation

    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

    Promoter

    Cbh

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-B19G

    Plasmid

    #59921

    Purpose

    Expression vector for Rabies virus SAD B19 glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP-TPD54

    Plasmid

    #172448

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJBL7010

    Plasmid

    #136942

    Purpose

    Constitutive sfGFP expression (high), bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. L4440_BioBrick-hsf-1-sgRNA-B

    Plasmid

    #177787

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hsf-1 (hsf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. TFORF3132

    Plasmid

    #144608

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESR2 (ESR2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. R777-E245 Hs.RASGRP2

    Plasmid

    #70529

    Purpose

    Gateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASGRP2 (RASGRP2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. TFORF3127

    Plasmid

    #144603

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIL3 (NFIL3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

    Plasmid

    #153003

    Purpose

    Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. dTomato-2xrGBD

    Plasmid

    #129625

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    dTomato-2xrGBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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