163,517 results

1. pET28.His.3C.Tau2N4R

   Plasmid

   #226396

   Purpose

   Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT Human, synthetic)
   

   Use

   Tags

   His.3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Use

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV delta R8.2

   Plasmid

   #12263

   Purpose

   First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

   Depositor

   Didier Trono

   Insert

   HIV-1 GAG/POL, Tat and Rev
   

   Use

   Lentiviral; Packaging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX303

   Plasmid

   #25897

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-tTRE-scFv-SunTag

   Plasmid

   #193138

   Purpose

   Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   scFv-SunTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-YFP

   Plasmid

   #13033

   Purpose

   Mammalian expression vector for cloning your gene fused to YFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Caspase1-flag

   Plasmid

   #21142

   Depositor

   Junying Yuan

   Article

   Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

   Insert

   caspase-1 (Casp1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. HIF-TRE-mStrawberry reporter

   Plasmid

   #158681

   Purpose

   This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM069

   Plasmid

   #216821

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold2
   

   Use

   Tags

   RIPGLIN and VinnyLinker

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVβ-p300-myc

    Plasmid

    #30489

    Purpose

    Mammalian expression of human p300 with myc tag

    Depositor

    Tso-Pang Yao

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ114

    Plasmid

    #85995

    Purpose

    One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified bovine U6-2

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXR002: EF1a-dCasRx-2A-EGFP

    Plasmid

    #109050

    Purpose

    Catalytically inactive dCasRx with 2A-EGFP

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. 1236 pcDNA3 Myr HA Akt3

    Plasmid

    #9017

    Depositor

    William Sellers

    Insert

    Akt3 (AKT3 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SLC16A1_STOP

    Plasmid

    #161100

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A1 (SLC16A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Use

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR_LG lenti-(empty)-del-CTS

    Plasmid

    #112895

    Purpose

    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

    Depositor

    Paul Blainey

    Article

    Feldman et al bioRxiv 262121

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

    Plasmid

    #231986

    Purpose

    Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    OVA-2A-ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hSyn-PSDΔ1,2-FRBx3

    Plasmid

    #225827

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    PSDΔ1,2-FRBx3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pH2B-mNG2(11)

    Plasmid

    #206043

    Purpose

    Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    H2B gene fused to the mNG2(11) fragment
    

    Use

    Synthetic Biology

    Tags

    H2B-mNG2(11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDMS151A_Tet-On-3G

    Plasmid

    #191577

    Purpose

    ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Tet-On(R) 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pC1-mitoRexYFP

    Plasmid

    #60246

    Purpose

    Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    mitoRexYFP
    

    Use

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-PARP1-3xFlag-WT

    Plasmid

    #111575

    Purpose

    Expresses full length PARP1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-TRE-dCas9-KRAB-MeCP2

    Plasmid

    #122267

    Purpose

    SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    dCas9-KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-EFS-foldon-GFP- PCP

    Plasmid

    #194503

    Purpose

    Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Insert

    foldon-GFP-PCP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid

    #50474

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EF.CMV.RFP

    Plasmid

    #17619

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cav1-GFP

    Plasmid

    #14433

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. UBC-4xmNG-dMCP

    Plasmid

    #231093

    Purpose

    Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods (2025). https://doi.org/10.1

    Insert

    4xmNG-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHR CD19-mMegf10 CAR

    Plasmid

    #113013

    Purpose

    for production of lentivirus encoding the CD19 CAR-Pmegf10

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Human, Synthetic, Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. T7-LacO-GFP_ColE1

    Plasmid

    #171648

    Purpose

    Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-GFPssrA-T7 Stop
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PEmax_2star_IVT

    Plasmid

    #227674

    Purpose

    plasmid for PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pEGFP-C1 F-tractin-EGFP

    Plasmid

    #58473

    Purpose

    Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    F-tractin (Itpka Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

    Plasmid

    #31476

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14 GFP donor EF1s I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    GFP has 14 amino acids deleted from c terminus

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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48. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)

    Plasmid

    #163505

    Purpose

    DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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50. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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