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Showing: 401 - 450 of 568 results

  2. GST-PBD

    Plasmid
    #60880
    Purpose
    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653
    Depositor
    Trina Schroer
    Article
    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1
    Insert
    Polo-like kinase 1 (PLK1 BC01486, Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid
    #227775
    Purpose
    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Article
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Inserts
    BFP-MCS
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70l
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    227775-plasmid-map-sequence-id-455547

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  9. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Beta actin
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134606-plasmid-map-sequence-id-258758

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  16. pCAG-memRFP

    Plasmid
    #224564
    Purpose
    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    Unspecified
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224564-plasmid-map-sequence-id-451482

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  20. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80067-plasmid-map-sequence-id-152621

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  21. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

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  22. pMTB2-Avi-Cerulean-RanGAP

    Plasmid
    #79878
    Purpose
    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79878-plasmid-map-sequence-id-149739

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  23. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid
    #79880
    Purpose
    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Use
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79880-plasmid-map-sequence-id-149770

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  24. pMTB2-RanGAP-Cerulean-Avi

    Plasmid
    #80068
    Purpose
    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Use
    Zebrafish biotagging
    Tags
    Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80068-plasmid-map-sequence-id-152622

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  25. cacnb1b pCAGGS

    Plasmid
    #206102
    Purpose
    expression of rat beta1b calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacnb1b (Cacnb1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/B-actin
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206102-plasmid-map-sequence-id-413014

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  31. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  38. pBSR (mCherry-surrogateTarget-bsr)

    Plasmid
    #66950
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    blasticidin
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    66950-plasmid-map-sequence-id-115273

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  40. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  41. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Expression
    Mutation
    Codon optimized
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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Showing: 401 - 450 of 568 results