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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,175 results

Showing: 4601 - 4650 of 19175 results

  1. pBS513 EF1alpha-cre

    Plasmid
    #11918
    Purpose
    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.
    Depositor
    Brian Sauer
    Article
    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.
    Insert
    cre (cre bacteriophage P1)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    11918-plasmid-map-sequence-id-163454

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  7. N174-MCS

    Plasmid
    #81061
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    81061-plasmid-map-sequence-id-359456

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  8. pcDNA3-Ii-OVA.I+II

    Plasmid
    #64596
    Purpose
    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation
    Depositor
    Martin Zenke, Sandra Diebold
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Use
    Tags
    Invariant chain
    Expression
    Mammalian
    Mutation
    Only amino acids 242-353 (class I and class II ep…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64596-plasmid-map-sequence-id-118017

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  13. mEmerald-CD9-10

    Plasmid
    #54029
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CD9 (CD9 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54029-plasmid-map-sequence-id-266657

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  15. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  17. pAAV.TBG.PI.Null.bGH

    Plasmid
    #105536
    Purpose
    empty AAV vector with TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    None
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105536-plasmid-map-sequence-id-202373

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  19. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  21. mCherry-Vimentin-N-18

    Plasmid
    #55158
    Purpose
    Localization: Intermediate Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55158-plasmid-map-sequence-id-267740

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  27. pcDNA3-Lyso-TORCAR

    Plasmid
    #64929
    Purpose
    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-TORCAR (LAMP1 Synthetic, Human)
    Use
    Tags
    Cerulean, Full-length LAMP1, and YPet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64929-plasmid-map-sequence-id-109691

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  40. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  50. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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Showing: 4601 - 4650 of 19175 results