Lentiviral Plasmids
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Why Lentivirus?
- Lentiviruses can transduce both actively proliferating and non-dividing cells. This makes lentiviruses very useful for studies in a variety of cell types including non-transformed cells.
- Lentivirus integrate their genetic material into the host cell allowing for stable, long-term expression of the transgene or shRNA.
Lentiviral Plasmids
| Addgene has assembled an extensive collection of lentiviral plasmids for a variety of applications. We have plasmids for cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, with fluorescent proteins, and more. Browse ALL lentiviral plasmids. |  |
Types of Lentiviral Plasmids:
Popular Lentiviral Plasmids
| Plasmid | Depositing Scientist | Generation | Type | Description |
|---|---|---|---|---|
| pLKO.1 puro | Bob Weinberg | 3rd | Transfer | U6-driven shRNA empty vector with puro resistance |
| pLKO.1 – TRC cloning vector | David Root | 3rd | Transfer | U6-driven shRNA empty vector; includes a stuffer for easy cloning |
| pSico | Tyler Jacks | 3rd | Transfer | Conditional (Cre-Lox), stable expression of shRNAs; addition of Cre TURNS ON shRNA expression |
| FUGW | David Baltimore | 3rd | Transfer | hUbC-driven EGFP; can be used for cDNA expresion |
| pLVTHM | Didier Trono | 2nd | Transfer | EF-1a driven-GFP and shRNA under the control of a tet responsive H1 promoter |
| pLJM1 | David Sabatini | 3rd | Transfer | CMV-driven EGFP; can be used for cDNA expression; puro resistance |
| pLionII | Garry Nolan | 3rd | Transfer | FIV-derived vector with a chimeric 5’LTR |
| pMD2.G | Didier Trono | N/A | Envelope | VSV-G expressing envelope vector |
| pCMV-VSV-G | Bob Weinberg | N/A | Envelope | VSV-G expressing envelope vector |
| pCI-VSVG | Garry Nolan | N/A | Envelope | VSV-G recommended for the FELIX (FIV-based) system |
| pCMV-dR8.2 dvpr | Bob Weinberg | 2nd | Packaging | Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd and 3rd generation transfer vectors |
| psPAX2 | Didier Trono | 2nd | Packaging | Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation transfer vectors |
| pRSV-Rev | Didier Trono | 3rd | Packaging | Contains Rev; can be used with 3rd generation transfer plasmids along with additional packaging components |
| pMDLg/pRRE | Didier Trono | 3rd | Packaging | Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev |