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20,802 results

Showing: 751 - 800 of 20802 results

  10. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  14. pAAV.GFAP.Cre.WPRE.hGH (AAV PHP.eB)

    Viral Prep
    #105550-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven expression of Cre. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    GFAP
    Available Since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100852-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (#100852). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRGECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV5)

    Viral Prep
    #105558-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV-Ef1a-fDIO mCherry (AAV Retrograde)

    Viral Prep
    #114471-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  23. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (AAV1)

    Viral Prep
    #51509-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE (#51509). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE plasmid DNA. hSyn-driven, Cre-dependent expression of cytoplasmic tdTomato and presynaptic EGFP (synaptophysin-fused). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    phSyn1
    Tags
    tdTomato (cytoplasmic, Cre-dependent), EGFP (presynaptic, C…
    Available Since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only

  26. pAAV-FLEX-tdTomato (AAV9)

    Viral Prep
    #28306-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  29. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

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  31. mCherry-NUP50-N-10

    Plasmid
    #55111
    Purpose
    Localization: Nuclear Pore, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    NUP50 (NUP50 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55111-plasmid-map-sequence-id-475625

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  35. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  40. pGP-AAV-syn-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #162376-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  41. pAAV-nEF-Coff/Fon-ChRmine-oScarlet (AAV8)

    Viral Prep
    #137160-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChRmine-oScarlet (#137160). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Flp (inhibited in the presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    oScarlet (Flp-dependent)
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  42. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162377-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  44. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  45. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV1)

    Viral Prep
    #26969-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  48. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Tags
    HA and Myr
    Expression
    Mammalian
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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Showing: 751 - 800 of 20802 results