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179,538 results

Showing: 8751 - 8800 of 179538 results

  2. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  6. pAAVS1-P-CAG-GFPluc2

    Plasmid
    #80493
    Purpose
    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
    Depositor
    Knut Woltjen
    Publication
    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPluc2
    Use
    Donor vector (human)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    80493-plasmid-map-sequence-id-151598

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  8. pAAV-hDlx-Flex-GFP-Fishell_6

    Plasmid
    #83895
    Purpose
    Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Publication
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has service
    AAV1, AAV2, AAV8, AAV9, and AAV Retrograde
    Insert
    eGFP
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    83895-plasmid-map-sequence-id-160320

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  12. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid
    #80408
    Purpose
    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells
    Depositor
    Michael Glotzer
    Publication
    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80408-plasmid-map-sequence-id-151385

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  15. pCDNA3-STAT3-S727D

    Plasmid
    #73364
    Purpose
    Expression of STAT3-S727D
    Depositor
    Jie Chen
    Publication
    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    Tags
    FLAG tagged
    Expression
    Mammalian
    Mutation
    E16K S727D
    Promoter
    CMV
    Available since
    May 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    73364-plasmid-map-sequence-id-423259

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  18. pEF5B-FRT-control-APEX

    Plasmid
    #73187
    Purpose
    Expresses "control"-APEX (cytosolic) in mammalian cells
    Depositor
    Maxence Nachury
    Publication
    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    control-APEX (Nphp3 Glycine max, Mouse)
    Tags
    internal GFP tag
    Expression
    Mammalian
    Mutation
    changed glycine 2 to alanine
    Promoter
    EF1alpha
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73187-plasmid-map-sequence-id-139537

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  24. Str-KDEL_SBP-mCherry-Ecadherin

    Plasmid
    #65287
    Purpose
    synchronize trafficking of Ecadherin from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ecadherin
    Tags
    IL-2 Signal Sequence and Streptavidin Binding Pro…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    65287-plasmid-map-sequence-id-120702

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  25. pCAGGS-FuG-E

    Plasmid
    #67509
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Publication
    Kato et al J Neurosci Methods. 2014 Apr 30;227:151-
    Insert
    Fusion Glycoprotein type E
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    67509-plasmid-map-sequence-id-118281

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  27. pcDNA3.1 iTEV

    Plasmid
    #64277
    Purpose
    Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)
    Depositor
    Xiaokun Shu
    Publication
    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Infrared fluorescent tobacco etch virus protease sensor (iTEV)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64277-plasmid-map-sequence-id-107780

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  30. pSBtet-BP

    Plasmid
    #60496
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    60496-plasmid-map-sequence-id-97551

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  31. pHL-EF1a-SphcCas9-iP-A

    Plasmid
    #60599
    Purpose
    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon usage optimized for human usage
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    60599-plasmid-map-sequence-id-97109

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  32. pCAG-B19L

    Plasmid
    #59922
    Purpose
    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene
    Depositor
    Ian Wickersham
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rabies virus RNA dependent RNA polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    59922-plasmid-map-sequence-id-94980

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  35. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    58807-plasmid-map-sequence-id-91105

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  36. mEmerald-Desmin-C-18

    Plasmid
    #54059
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Desmin (DES Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    54059-plasmid-map-sequence-id-261656

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  38. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    54537-plasmid-map-sequence-id-127234

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  43. GI-Gal4 DBD

    Plasmid
    #42500
    Purpose
    GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Publication
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GIGANTEA fused with Gal4 DBD
    Tags
    Gal4 DNA binding domain (DBD)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    42500-plasmid-map-sequence-id-57340

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  47. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    10 Articles

  48. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid
    #35509
    Purpose
    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Karl Deisseroth
    Publication
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Has service
    AAV5 and AAV9
    Insert
    hChR2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    35509-plasmid-map-sequence-id-196784

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Showing: 8751 - 8800 of 179538 results