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Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-BSD-tet-mEGFP-MIB1
Plasmid#140241PurposeLentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatmentDepositorInsertTet-inducible mEGFP-MIB1 (MIB1 Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterTREAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-mCherry
Plasmid#217005Purposelentiviral plasmid for gRNA cloning with selectable mCherry expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSlow-FT-N1
Plasmid#31912DepositorInsertSlow-FT
UseTagsExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCasRx-ccdB
Plasmid#221003PurposeDestination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ESRRG_P2A_Hygro_Barcode
Plasmid#120435PurposeBarcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertESRRG (ESRRG Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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GluN2A (px330:2-guideCas9)
Plasmid#169428PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd nog
Plasmid#115488PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
Plasmid#37120PurposeDO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cellsDepositorInsertTdTomato-EGFP
UseAAV and Cre/Lox; Cre-switchTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG
Plasmid#159978PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-TRAF1
Plasmid#111199Purposefluorescent fusion proteinDepositorInsertTRAF1 (TRAF1 Human)
UseTagsEGFP-FLAGExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee RunxDN mCherry
Plasmid#80158Purposeretroviral expression of Runx1DN and mCherryDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHA and IRES-mCherryExpressionMammalianMutationDN--truncated version of the Runx1 protein contai…PromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-mScarlet/CAAX
Plasmid#197592PurposeLentiviral transfer vector for the neuronal expression of membrane-bound mScarlet (good visualization of neuronal processes)DepositorInsertmScarlet-CAAX
UseLentiviralTagsCAAXExpressionMutationPromoterhSynIAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-VPS15
Plasmid#99326PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertVPS15 (PIK3R4 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-AuroraAKD-C-7
Plasmid#54998PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertAuroraA KD (AURKA Human)
UseTagsmCherryExpressionMammalianMutationKinase Dead - I57V, K162RPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-DIO-JEDI-2P-WPRE (AAV1)
Viral Prep#179460-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-WPRE (#179460). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-WPRE plasmid DNA. EF1a-driven, Cre-dependent expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagscEGFP (Cre-dependent)Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCAM-GFP
Plasmid#214475PurposeLentiviral vector for the expression of MCAM-GFP fusion protein in mammalian cells.DepositorInsertMCAM (MCAM Human)
UseLentiviral; GatewayTagsEGFPExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1alpha
Plasmid#17652DepositorInsertHP1 alpha (CBX5 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP1-S127A
Plasmid#17790DepositorInsertYes-kinase associated protein mutant S127A (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationSerine 127 to AlaninePromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
SPECS library
Pooled Library#127842PurposeFor performing high-throughput cell state-specific synthetic promoter screeningDepositorSpeciesSyntheticUseLentiviralAvailable sinceAug. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPKAPK2
Plasmid#23426DepositorInsertMAPKAPK2 (MAPKAPK2 Human)
UseGateway donor vectorTagsExpressionMutationP18APromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified) (AAV5)
Viral Prep#59462-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (codon diversified)Available sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPKAPK3
Plasmid#23790DepositorInsertMAPKAPK3 (MAPKAPK3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEMJ_042
Plasmid#214061PurposeT7 inducible LanTERN (pRSET)DepositorInsertLanTERN
UseTags6x His, T7 Tag, and Xpress(tm) TagExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
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GluN2B (px330:2-guideCas9)
Plasmid#169430PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.S72A
Plasmid#106200PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72APromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC17A5_STOP
Plasmid#161102PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC17A5 (SLC17A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGCN2-FLAG-IP
Plasmid#101794PurposeRetroviral vector encoding C-terminally FLAG tagged mouse GCN2DepositorInsertGcn2 (Eif2ak4 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry (AAV Retrograde)
Viral Prep#114470-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherryAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)
Viral Prep#192552-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(-KTS)
Plasmid#220565PurposeLentiviral expression of human EWSR1::WT1(-KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0509
Plasmid#143036PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTSHZ2 (TSHZ2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Plasmid#26969PurposeAAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationHistidine 134 is mutated to ArgininePromoterCaMKIIaAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 GFP shRNA
Plasmid#30323DepositorInsertGFP shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag pLJM1 RagD 121L
Plasmid#19318DepositorInsertRas-related GTP binding D (RRAGD Human)
UseLentiviralTagsFlagExpressionMammalianMutationQ121LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA 3.1 AURKA Lys162Met
Plasmid#157751PurposeExpression of kinase-dead AURKA K62M in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsExpressionMammalianMutationAURKA K162M is a kinase-dead version of AURKAPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-miniTurbo-V5-STOP_IDG-K
Plasmid#158158PurposeGateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-miniTurbo-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD3
Plasmid#226405PurposeExpression of human MOSPD3 fused to EGFP in mammalian cellsDepositorInsertMOSPD3 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only