179,538 results
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Plasmid#11793DepositorInsertNFAT2 (Nfatc1 Mouse)
ExpressionBacterialMutationConstitutively active: multiple serine to alanine…Available sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p210 pCMV-CREM
Plasmid#8395PurposeCre with chimeric/β-globin intron within the Cre coding sequenceDepositorInsertCREM
UseCre/LoxExpressionMammalianMutationmodified CrePromoterCMVAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MT-cdc20
Plasmid#11593DepositorAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM596
Plasmid#8837DepositorPublicationTypeEmpty backboneTagsE. coli MBPExpressionBacterialAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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p4028 GST-E6AP
Plasmid#8655DepositorAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TO-MYOD1-shOct4
Plasmid#182309PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationshOct4PromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (AAV8)
Viral prep#128589-AAV8PurposeReady-to-use AAV8 particles produced from AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] (#128589). In addition to the viral particles, you will also receive purified AAV-Syn-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. Syn-driven, Flp-dependent ChrimsonR-tdTomato expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomatoAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SomaGCaMP6f2 (AAV9)
Viral prep#158757-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG promoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 E
Plasmid#158080PurposeExpresses SARS-CoV-2 envelope protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 envelope (E )
ExpressionMammalianAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163055PurposeGrx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PE2-C
Plasmid#164908PurposeExpresses the C-terminal split-intein fragment of PE2DepositorInsertPE2-C
UseAAVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-eGFP-C1-VPS35
Plasmid#163622Purposehuman VPS35 cDNA C-terminally tagged with eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman VPS35 (VPS35 Human)
TagsGFPExpressionMammalianAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP His8
Plasmid#160687PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP His8ExpressionMammalianPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)
Plasmid#140534PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOsTIR1(F74G) mAID-EGFP-NLS TOL2
ExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWPI-IRES-Bla-Ak-ACE2-TMPRSS2
Plasmid#154983PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRExpressionMammalianAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mDlx-GFP-Fishell-1 (AAV Retrograde)
Viral prep#83900-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsGFPAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-mCherry-cNLS
Plasmid#138527PurposeInducible expression of mCherry with nuclear localization signalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-Citron1
Plasmid#134305Purposemitochondria-targeted direct-response citrate sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCitron1
ExpressionMammalianPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2-TetOminiCMV-OSK
Plasmid#136552PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas serviceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-TAG-NICD
Plasmid#130934PurposePiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cellsDepositorAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDuet-sumo-h-cGAS
Plasmid#127161Purposeexpression of human cGAS proteinDepositorAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVExpressionMammalianAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE Tubb3-GFP KI
Plasmid#131497PurposeEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneTagsGSTExpressionMammalianAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBLO 62.5
Plasmid#123124PurposeHuman expression vector for planctomyces CasX and sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCasX protein
CasX sgRNA
UseCRISPRExpressionMammalianAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
TagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas serviceAAV1, AAV5, AAV8, AAV9, and AAV RetrogradeInsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
TagsMBPExpressionBacterialPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1 EGFP-Tau
Plasmid#108868PurposeExpresses EGFP linked to human TauDepositorInsertTau (MAPT Human)
UseLentiviralTagsEGFPExpressionMammalianMutationhuman four-repeat tau lacking the N-terminal sequ…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZyxin-mMaple3
Plasmid#101151PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized mMaple3 fused to zyxin
ExpressionMammalianPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-EYFP
Plasmid#104052PurposeAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoterDepositorHas serviceAAV PHP.V1InsertEYFP
UseAAVPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSC101_TIMER
Plasmid#103057Purposelow copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
ExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
TagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40
Plasmid#101064PurposeAAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2_L398T
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AID-BE3
Plasmid#100803PurposeExpresses AID-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertactivation induced cytidine deaminase (AICDA Human, S. pyogenes)
ExpressionMammalianPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-mRuby2
Plasmid#99123PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid kit#1000000115PurposeA collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorApplicationStrain CollectionsVector typeBacterial ExpressionAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only