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179,463 results

Showing: 151 - 200 of 179463 results

  2. pZac2.1 gfaABC1D-cyto-GCaMP6f (AAV5)

    Viral prep
    #52925-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP6f (#52925). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) cyto-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Publication
    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV1)

    Viral prep
    #100837-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29 Articles
    72247-plasmid-map-sequence-id-485302

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  10. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Publication
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36 Articles
    123461-plasmid-map-sequence-id-417805

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  12. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84 Articles
    89308-plasmid-map-sequence-id-425605

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  15. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185 Articles
    12254-plasmid-map-sequence-id-485802

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  16. Human sgRNA library Brunello in lentiGuide-Puro

    Pooled library
    #73178
    Purpose
    Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Has service
    Lentiviral Prep
    Expression
    Mammalian
    Use
    CRISPR and Lentiviral
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160 Articles

  19. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58 Articles
    99154-plasmid-map-sequence-id-352449

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  24. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    75112-plasmid-map-sequence-id-305764

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  25. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Insert
    Monomer Red Fluorescent Protein
    Expression
    Mammalian
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48 Articles
    13032-plasmid-map-sequence-id-211651

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  28. psPAX2-D64V

    Plasmid
    #63586
    Purpose
    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
    Depositor
    David Rawlings, Andrew Scharenberg
    Publication
    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi
    Insert
    Packaging plasmid for integrase deficient lentiviral vectors
    Use
    Integrase deficient lentiviral packaging vector
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    63586-plasmid-map-sequence-id-464218

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  32. mCherry2-N1

    Plasmid
    #54517
    Purpose
    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    54517-plasmid-map-sequence-id-352457

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  35. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Publication
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Promoter
    CMV
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44 Articles
    52659-plasmid-map-sequence-id-430007

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  36. pAAV-CAG-GFP (AAV Retrograde)

    Viral prep
    #37825-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  37. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Publication
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    17870-plasmid-map-sequence-id-520532

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  46. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    57 Articles
    44248-plasmid-map-sequence-id-190219

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  48. pcDNA3-YFP

    Plasmid
    #13033
    Purpose
    Mammalian expression vector for cloning your gene fused to YFP
    Depositor
    Doug Golenbock
    Insert
    Yellow fluorescent protein
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    33 Articles
    13033-plasmid-map-sequence-id-210331

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Showing: 151 - 200 of 179463 results