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20,802 results

Showing: 251 - 300 of 20802 results

  13. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  16. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available Since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138272-plasmid-map-sequence-id-273617

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  22. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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  23. mEmerald-FilaminA-N-9

    Plasmid
    #54098
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54098-plasmid-map-sequence-id-491758

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  25. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  26. pcDNA3-TfR-OVA

    Plasmid
    #64600
    Purpose
    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation
    Depositor
    Sandra Diebold, Martin Zenke
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Tags
    Transmembrane domain of Transferrin Receptor (TfR…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64600-plasmid-map-sequence-id-117969

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  33. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  39. pMAL-p5X

    Plasmid
    #150814
    Purpose
    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
    Depositor
    Paul Riggs
    Type
    Empty backbone
    Tags
    malE A313V
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 22, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    150814-plasmid-map-sequence-id-306372

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  43. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  49. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Charles Hunter
    Article
    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126072-plasmid-map-sequence-id-247528

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Showing: 251 - 300 of 20802 results