76,289 results

1. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209724

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1_GEM4-Halo-FRB

   Plasmid

   #197057

   Purpose

   Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRosa26-DEST

   Plasmid

   #21189

   Purpose

   Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

   Depositor

   Peter Hohenstein, Nick Hastie

   Article

   Hohenstein et al Pathogenetics. 2008 . 1(1):3.

   Insert

   GateWay conversion cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-N-Luc

   Plasmid

   #185357

   Purpose

   For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).

   Depositor

   Ayaz Najafov

   Insert

   Firefly Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Use

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS6-YFP-SMASh

   Plasmid

   #68853

   Purpose

   Expresses SMASh tag fused to YFP at C terminus in mammalian cells

   Depositor

   Michael Lin

   Article

   Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

   Insert

   YFP-SMASh
   

   Use

   Tags

   SMASh tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-ApplePy1Highest

   Plasmid

   #239064

   Purpose

   Mammalian expression of highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

   Depositor

   Robert Campbell

   Article

   Imai et al bioRxiv 2025.04.17.649293

   Insert

   ApplePy1Highest
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 HA-PHLPP1 full length

   Plasmid

   #37100

   Purpose

   Expresses HA tagged, full-length human PHLPP1 in mammalian cells

   Depositor

   Alexandra Newton

   Article

   Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

   Insert

   PHLPP1 (PHLPP1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mito-mGreenLantern

    Plasmid

    #164461

    Purpose

    Localization of mGreenLantern fluorescent protein to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid

    #172508

    Purpose

    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF5/FRT/DEST-3xHA/mGli3/Flag

    Plasmid

    #51246

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. AAVS1_Puro_hPGK1_EBFP_Donor

    Plasmid

    #178089

    Purpose

    HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EBFP (T65S/F145Y)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PBCAG-mRFP

    Plasmid

    #40996

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Use

    Tags

    GCaMP3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CAG sHRPa-NRX

    Plasmid

    #73143

    Purpose

    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-NRX
    

    Use

    Tags

    2x HA tag and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-PIK3CD

    Plasmid

    #174190

    Purpose

    Expresses PIK3CD in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.4_StrepII_TEV_PP2Ac

    Plasmid

    #217477

    Purpose

    Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    PP2Ac
    

    Use

    Tags

    StrepII, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBiFC-bFosVC155

    Plasmid

    #22013

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Oct. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBOBI-FLuc

    Plasmid

    #170674

    Purpose

    Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    luc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGLS3-5xHRE-p53(K164R)

    Plasmid

    #72555

    Purpose

    Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    K164R

    Promoter

    E1B minimal promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CAG sHRPb-NLG

    Plasmid

    #73144

    Purpose

    Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA 3.1 SIRT1-FL

    Plasmid

    #105670

    Purpose

    Mammalian expression construct of mouse SIRT1 full length isoform

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEG BacMam C term GFP His8

    Plasmid

    #160681

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    His8 GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-HA-USP5

    Plasmid

    #22590

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP5 (USP5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-mCh-Cry2olig

    Plasmid

    #101222

    Purpose

    Cry2olig(E490G)

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2olig
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TRE-SEAP

    Plasmid

    #210511

    Purpose

    SEAP reporter vector for LAUNCHER system

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    secreted alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pf hPGK mc2 IRES puro

    Plasmid

    #206237

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Use

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mCherry-Rab5C-S35N

    Plasmid

    #110503

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CTCF-C CRISPR

    Plasmid

    #140647

    Purpose

    CTCF tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEX-CMV-SP-YFP-STM2(15-746)

    Plasmid

    #18862

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    Stromal interaction molecule 2 (STIM2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP inserted between signal peptide(SP) of STIM1 …

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. CAG-AausFP1-Z1

    Plasmid

    #135047

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAS088

    Plasmid

    #211502

    Purpose

    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pmCherry-Akt1(WT)

    Plasmid

    #86631

    Purpose

    Expresses mCherry-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

47. pDESTmycIGF2BP3

    Plasmid

    #19879

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Use

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMRX-INU-SEC62-FLAG

    Plasmid

    #128263

    Purpose

    Expresses SEC62 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    SEC62 (SEC62 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pIGneo

    Plasmid

    #52116

    Purpose

    a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge