Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

75,759 results

Showing: 1501 - 1550 of 75759 results

  6. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

    Plasmid
    #221352
    Purpose
    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221352-plasmid-map-sequence-id-436798

    Enlarge

  7. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa

    Plasmid
    #221354
    Purpose
    Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221354-plasmid-map-sequence-id-436804

    Enlarge

  8. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC

    Plasmid
    #221351
    Purpose
    Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221351-plasmid-map-sequence-id-436779

    Enlarge

  10. TFORF3455

    Plasmid
    #144931
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF513 (ZNF513 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144931-plasmid-map-sequence-id-404505

    Enlarge

  11. TFORF0088

    Plasmid
    #142455
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF408 (ZNF408 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142455-plasmid-map-sequence-id-397222

    Enlarge

  12. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid
    #140271
    Purpose
    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    140271-plasmid-map-sequence-id-317910

    Enlarge

  16. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

    Enlarge

  17. Lamp1-mScarlet-I

    Plasmid
    #98827
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98827-plasmid-map-sequence-id-430619

    Enlarge

  27. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

    Enlarge

  32. pmScarlet3-H_I3

    Plasmid
    #239343
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Insert
    I3-01
    Use
    Tags
    mScarlet3-H (a.k.a. mYongHong)
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239343-plasmid-map-sequence-id-474361

    Enlarge

  38. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid
    #197501
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197501-plasmid-map-sequence-id-386690

    Enlarge

  40. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Use
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

    Enlarge

  44. pcDNA3.1-(-)-Pur

    Plasmid
    #200458
    Purpose
    Eukaryotic expression vector
    Depositor
    Bertrand Collet
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200458-plasmid-map-sequence-id-394118

    Enlarge

Showing: 1501 - 1550 of 75759 results