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20,197 results

Showing: 4161 - 4200 of 20197 results

  2. pLXSN16E7

    Plasmid
    #52396
    Purpose
    The plasmid, pLXSN16E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E7 gene.
    Depositor
    Denise Galloway
    Article
    Halbert et al J Virol. 1991 Jan;65(1):473-8.
    Insert
    HPV 16E7
    Use
    Retroviral
    Available Since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52396-plasmid-map-sequence-id-81851

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  10. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  17. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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  21. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid
    #61593
    Purpose
    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
    Depositor
    Feng Zhang
    Article
    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    HA, NLS, and OLLAS tag
    Expression
    Mammalian
    Mutation
    K175R and K736R (deUb mutations)
    Available Since
    Feb. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61593-plasmid-map-sequence-id-470051

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  27. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

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  30. pCS2TAL3-DD

    Plasmid
    #37275
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    Flag, Fok I DD, Tal-C, and Tal-N
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37275-plasmid-map-sequence-id-46872

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Showing: 4161 - 4200 of 20197 results