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8,636 results

Showing: 6251 - 6300 of 8636 results

  3. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  8. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  9. pAAV-GFAP-hChR2(H134R)-EYFP

    Plasmid
    #27054
    Purpose
    AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    GFAP
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27054-plasmid-map-sequence-id-12795

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  16. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141273-plasmid-map-sequence-id-288100

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  20. pBOB-CAG-SARS-CoV2-Spike D614G-HA

    Plasmid
    #158761
    Purpose
    3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Spike D614G
    Promoter
    CAG
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158761-plasmid-map-sequence-id-309514

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  31. CMV-EGFP-ssHRP-TM

    Plasmid
    #31156
    Depositor
    Hollis Cline
    Article
    Li et al Front Neural Circuits. 2010 . 4():6.
    Insert
    ssHRP-TM
    Use
    Membrane targeted hrp (mhrp)
    Tags
    Expression
    Mammalian
    Mutation
    includes signal sequence and a transmembrane doma…
    Promoter
    CMV promoter including immediate early promoter/e…
    Available since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. pCMVMagFRET-1

    Plasmid
    #50742
    Purpose
    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Insert
    gene encoding for MagFRET-1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50742-plasmid-map-sequence-id-327713

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  36. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Promoter
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  38. GFP-BLM

    Plasmid
    #80070
    Purpose
    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)
    Depositor
    Nathan Ellis
    Article
    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98
    Insert
    BLM (BLM Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    If correct ID, the cDNA is from HeLa and has char…
    Promoter
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80070-plasmid-map-sequence-id-423157

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  48. pENTR11 WT NFAT1

    Plasmid
    #11791
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    11791-plasmid-map-sequence-id-7060

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  50. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Article
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105627-plasmid-map-sequence-id-207217

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Showing: 6251 - 6300 of 8636 results