4,124 results

1. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Stephen Elledge, Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mTagBFP2-pBAD

   Plasmid

   #54572

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, T7, and mTagBFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSI-Check2-hRluc-NFkB-firefly

   Plasmid

   #106979

   Purpose

   Luciferase reporter with internal control for NFkB signal.

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   NFKB recognition sites (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EF.STAT3C.Ubc.GFP

   Plasmid

   #24983

   Purpose

   3rd generation lentiviral expression of constitutively active Stat3

   Depositor

   Linzhao Cheng

   Article

   Hillion et al Cancer Research 68, 10121, December 2008

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A662C, N664C (constitutively active)

   Promoter

   Available since

   May 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-M-puro

   Plasmid

   #125839

   Purpose

   modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1-Tet-OsTIR1(F74G)-V5

   Plasmid

   #158664

   Purpose

   Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(F74G) AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   F74G

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pimEJ5GFP

   Plasmid

   #44026

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ5GFP egfp-based chromosomal break reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pCAGGS promoter and eGFP separated by pgkPURO cas…

   Promoter

   pCAGGS

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ODD-Luciferase-pcDNA3

   Plasmid

   #18965

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   ODD-Firefly Luciferase (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HIF1a ODD sequence fused in frame to luciferase O…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. beta-arrestin2 mYFP

    Plasmid

    #36917

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 7TFP

    Plasmid

    #24308

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAVS1-TALEN-L

    Plasmid

    #59025

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-L
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1-TALEN-R

    Plasmid

    #59026

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-R
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid

    #102245

    Purpose

    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

    Depositor

    Jacob Corn

    Insert

    dCas9-VPR-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-VPR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH-CMV-mCherry-T2A-Puro

    Plasmid

    #72264

    Purpose

    Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

    Depositor

    Kazuhiro Oka

    Insert

    mCherry-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CD3 delta YFP

    Plasmid

    #11951

    Depositor

    Nico Dantuma

    Article

    Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

    Insert

    CD3delta-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xAP1pGL3 (3xAP-1 in pGL3-basic)

    Plasmid

    #40342

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    3xAP-1
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains three canonical AP-1 binding sites (TGAC…

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PH-PLCD1-GFP

    Plasmid

    #51407

    Purpose

    Is a biosensor for PI(4,5)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PGKneotpAlox2

    Plasmid

    #13444

    Depositor

    Philippe Soriano

    Article

    Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

    Insert

    PGKneotpAlox2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAdTrack-CMV

    Plasmid

    #16405

    Purpose

    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-EF1a-IRES-Blast

    Plasmid

    #85133

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pShuttle-CMV

    Plasmid

    #16403

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. T7-VEE-GFP

    Plasmid

    #58977

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSin Helper

    Plasmid

    #127694

    Purpose

    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Glycoproteins
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

37. pDx_mScarlet3

    Plasmid

    #189754

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-2xFLAG-SREBP-2

    Plasmid

    #26807

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    L7V, G76S, K395R and I482L

    Promoter

    CMV

    Available since

    July 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTSin EGFP

    Plasmid

    #127695

    Purpose

    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge