We narrowed to 18,983 results for: INO

1. pLPC-S22A-Progerin

   Plasmid

   #69065

   Purpose

   encodes a Progerin mutant where serine 22 is mutated to an alanine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Progerin with serine 22 mutated to alanine

   Promoter

   CMV

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCIneoGFP-BMI1

   Plasmid

   #128328

   Purpose

   Mammalian expression vector of GFP-tagged human BMI1

   Depositor

   Yutaka Hata

   Article

   Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

   Insert

   BMI1 (BMI1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

   Plasmid

   #9031

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV_EF1a-NAE1_IRES_Puro

   Plasmid

   #235659

   Purpose

   human NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)

   Depositor

   Lorenz Studer

   Article

   Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

   Insert

   NAE1 (NAE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208041

   Purpose

   Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   TurboID
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 SARS-CoV-2 S D614

   Plasmid

   #158074

   Purpose

   Expresses SARS-CoV-2 spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

   Plasmid

   #164827

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   miniCMV and pGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

   Plasmid

   #164828

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   miniCMV and pGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-GFP-IMP2-2

   Plasmid

   #42175

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OA-1136J

    Plasmid

    #153023

    Purpose

    Express His-MBP-TEV-CasRx recombinant protein

    Depositor

    Omar Akbari

    Article

    Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

    Insert

    CasRx
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 902 pLNCX myr HA Akt1 K179M

    Plasmid

    #9006

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    K179M. kinase dead.

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid

    #227106

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1011

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_AR-RE_MLPmin_BC1014-luc2

    Plasmid

    #227108

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1014

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Zeo_BACE1

    Plasmid

    #227105

    Purpose

    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_ER-RE_MLPmin_BC1063-luc2

    Plasmid

    #227109

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAcBac1-BacMam-4x-PyOtR

    Plasmid

    #217364

    Purpose

    Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    4 copies of pyrrolysyl-tRNA mutant PyOtR
    

    Use

    Baculoviral

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pDUET-1-alpha-synuclein-mCerulean3-His6

    Plasmid

    #110061

    Purpose

    For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    6 x His and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-SopB

    Plasmid

    #183657

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5 Myc-tagged PSK

    Plasmid

    #197149

    Purpose

    Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTwist-WIV1-CoV Δ18

    Plasmid

    #164439

    Purpose

    Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    WIV1-CoV Spike truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Use

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. STARR-seq luciferase INF enhancer vector_ORI_IFIT2

    Plasmid

    #99321

    Purpose

    Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr10: 91060605-91062447
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hIL23p19 pEF6-V5 (MJS_004)

    Plasmid

    #72492

    Purpose

    Expression of human IL23p19 in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    IL23p19 (IL23A Human)
    

    Use

    Tags

    His/V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR-myr-AKT-HA

    Plasmid

    #31790

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Entry vector

    Tags

    HA and Myr

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pKE13-ISceI-fabp10a:CreERT2

    Plasmid

    #198242

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTwist-SARS-CoV-2 Δ18 B.1.351v2

    Plasmid

    #169463

    Purpose

    Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-rBrokenheart

    Plasmid

    #203394

    Purpose

    AAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    Brokenheart
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-RNF51

    Plasmid

    #126546

    Purpose

    Expresses GFP-tagged RNF51 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF51 (BMI1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTwist-SARS-CoV-2 Δ18 B.1.1.7

    Plasmid

    #169459

    Purpose

    Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. W118-1_hZBTB7B

    Plasmid

    #180380

    Purpose

    lentiviral transduction of human ZBTB7B gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    ZBTB7B (ZBTB7B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry

    Plasmid

    #202818

    Purpose

    Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    addition of a dual aptamer RNA tag 5' to the…

    Promoter

    U6

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV_ER-RE_MLPmin_BC1065-luc2

    Plasmid

    #227111

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV_GR-RE_MLPmin_BC1094-luc2

    Plasmid

    #227114

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEMS2214

    Plasmid

    #158417

    Purpose

    AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple265 (PCP2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple265

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pCLHCX-mPM20D1-flag

    Plasmid

    #84566

    Purpose

    mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

    Depositor

    Bruce Spiegelman

    Article

    Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

    Insert

    peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    Retroviral

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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43. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple349

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    A662C N664C

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. 1017 pcDNA3 Myr Akt1 deltaPH

    Plasmid

    #9009

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 dPH (AKT1 Human)
    

    Use

    Tags

    myr (MGSSKSKPKDPSQR)

    Expression

    Mammalian

    Mutation

    delta PH domain (aa1-130)

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

47. hRAD18 dC2-EGFP

    Plasmid

    #68826

    Purpose

    Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

    Depositor

    Satoshi Tateishi

    Article

    Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

    Insert

    human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pTwist-SARS-CoV-2 Δ18 B.1.351v3

    Plasmid

    #169464

    Purpose

    Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. Flag Leucyl tRNA synthetase pRK5

    Plasmid

    #46341

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    leucyl tRNA synthetase (LARS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid

    #108546

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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