162,503 results

1. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWN_U6-B2M-miniGag-Cas9

   Plasmid

   #228958

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-CFP II (pMIC II)

   Plasmid

   #52109

   Purpose

   a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-dLight1.5

   Plasmid

   #111058

   Purpose

   Expresses dLight1.5 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.5
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEP4 E02S ET2K

   Plasmid

   #20927

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT85_PS-mEos3.2_GentaR

   Plasmid

   #173895

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Spiralin

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPICZaa-STE2-cohis

   Plasmid

   #133433

   Purpose

   Yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

   Depositor

   Gerhard Wagner

   Insert

   STE2 (STE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C59S/C252A, Codon optimized for P. pastoris, and …

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0335

    Plasmid

    #144358

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF91 (ZNF91 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ADRB1-Tango

    Plasmid

    #66219

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADRB1 (ADRB1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTipi2.1_Anti-Rho IPI-[1D4] Mouse/Rabbit HC

    Plasmid

    #221365

    Purpose

    Mammalian expression of Anti-Rho IPI-[1D4] Mouse/Rabbit HC; to be used with Anti-Rho IPI-[1D4] light chain (Plasmid 221366) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Rho IPI-[1D4] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

    Plasmid

    #221366

    Purpose

    Mammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pXL002-ishRNA-beta-catenin-1

    Plasmid

    #36297

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of beta-catenin-1 (CTNNB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

16. T-2A-EGFP-PGK-Puro

    Plasmid

    #83344

    Purpose

    Donor vector to knocin EGFP into human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    Human Brachyury knockin homology arms (TBXT Human)
    

    Use

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF3448

    Plasmid

    #144924

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP63 (TP63 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Expression

    Mutation

    Promoter

    CMV-MIE

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Synthetic, Mouse)
    

    Use

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Mutation

    Promoter

    TET

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF2260

    Plasmid

    #141998

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFLP-hyg

    Plasmid

    #87831

    Purpose

    Expresses FLP recombinase with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    heat-shock inducible

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. P2A-NLS-tdtomato-EPG backbone

    Plasmid

    #215548

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLJM1-FLAG-GFP-OSBP

    Plasmid

    #134659

    Purpose

    Flag and GFP-tagged OSBP

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. M77 pIRESpuro-GLUE-hDsh3

    Plasmid

    #15102

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    Dsh3 (DVL3 Human)
    

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Use

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mTagBFP2-Farnesyl-5

    Plasmid

    #55295

    Purpose

    Localization: Plasma Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. iLID-mCl3-MAP7N

    Plasmid

    #190167

    Purpose

    Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    iLID-mClover3-MAP7N
    

    Use

    Tags

    iLID-mClover3

    Expression

    Mammalian

    Mutation

    aa 1-309 from human MAP7 delta175-213

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Green Glifon600

    Plasmid

    #126207

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon600
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-ABEmax-NRCH

    Plasmid

    #136923

    Purpose

    Expresses ABEmax-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid

    #148120

    Purpose

    Mammalian Expression of HshnRNPH1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPH1 (HNRNPH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAV-CAG-Sun1-GFP-WPRE-BC2-pA

    Plasmid

    #220754

    Purpose

    Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    Sun1-GFP-WPRE-BC2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAV-CAG-Sun1-GFP-WPRE-BC1-pA

    Plasmid

    #220753

    Purpose

    Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    Sun1-GFP-WPRE-BC1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mPA-GFP-LaminB1-10

    Plasmid

    #57141

    Purpose

    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pAL-rfp_T

    Plasmid

    #74075

    Purpose

    pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA rfp (T)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. V5-ERG-R367K

    Plasmid

    #118624

    Purpose

    Expresses V5 tagged ERG R367K (DNA binding mutant) protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (R367K DNA binding mutant) (ERG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R367K

    Promoter

    EF1a

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBPGUw

    Plasmid

    #17575

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Tools for neuroanatomy and neurogenetics

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRSET-MagLOV

    Plasmid

    #219957

    Purpose

    Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

    Depositor

    Andrew York

    Article

    Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

    Insert

    MagLOV
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    AsLOV2 C450P L496V Q513K G528K D540M

    Promoter

    T7

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMT85_P438-mEOS3.2_GentaR

    Plasmid

    #173894

    Purpose

    Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

    Depositor

    Yonathan Arfi

    Article

    Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

    Insert

    mEos3.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P438

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAAV-Bcl2

    Plasmid

    #195551

    Purpose

    Expresses Bcl2 in mammalian cells

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CoV2-Spike-D614G

    Plasmid

    #177960

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. p2xFlag CMV2-YAP2-1st WW mutant

    Plasmid

    #19046

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein isoform 3 (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHAGE-DDX3X

    Plasmid

    #116730

    Purpose

    Lentiviral expression of DDX3X

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge