We narrowed to 6,273 results for: nls
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Plasmid#205170PurposeExpresses NLS-mCherry-FIREtag in mammalian cellsDepositorInsertNLS-mCherry-FIREtag
ExpressionMammalianAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-AausFP1
Plasmid#191096PurposeTo express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-AausFP1
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xPCP-2xGFP
Plasmid#71243PurposeUsed to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.DepositorInsert2xPCP-2xGFP
UseP element-mediated germline transformationTagsEGFPExpressionInsectPromoterHsp83Available SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UbC NLS-HA-MCP-YFP
Plasmid#31230DepositorInsertNLS-HA-MCP-YFP
UseLentiviralExpressionMammalianAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 RPEL1-EGFP-3XNLS
Plasmid#58469PurposeExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoterDepositorInsertsTagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-CRY2-NLS
Plasmid#145259PurposeMammalian Expression of Nuclear Control OptoConstruct with SV40 NLSDepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-MCS-NLS-3XFLAG-V5
Plasmid#196383PurposeEmpty backbone vector to insert cDNA for strong expression in mammalian cellsDepositorTypeEmpty backboneTags3xFLAG and V5ExpressionBacterial and MammalianPromoterCAGGsAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-NLS-SNAP
Plasmid#174879PurposePiggyBac transposon vector for expression of NLS-SNAPDepositorInsertNLS-SNAP
UsePiggybac vectorExpressionMammalianAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertGFP
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGsAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-PhoCl-mCherry
Plasmid#87691PurposeExpress NLS-PhoCl-mCherry in mammalian cellsDepositorInsertNLS-PhoCl-mCherry
Tagsnuclear localization signalExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AID∆C-Cas9n-UGI-NLS
Plasmid#198890PurposeExpress AID(1-188) BE3-based base editorDepositorInsertActication induced deaminase (AICDA Human)
TagsNLSExpressionMammalianMutationDeletion of amino acids 189-198PromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-E2-Crimson-NLS
Plasmid#125263PurposeFluorescent reporter for cell sizeDepositorInsertE2-Crimson-NLS
UseLentiviralTagsNLSExpressionMammalianPromoterEF1aAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-NLS-TPP1
Plasmid#53585PurposeMammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.DepositorInsertTPP1 (ACD Human)
Tags3xFLAG and NLSExpressionMammalianMutationSilent mutation to eliminate PacI sitePromoterCMVAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3Ai-Cas9n-UGI-NLS
Plasmid#109425PurposeExpresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-ICUE3-NLS
Plasmid#138255PurposeFRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertFKBP-ICUE3-NLS
TagsFKBP, Nuclear localization signal (NLS), cpVenus[…ExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS887_pTetR-P2A-BFPnls/sgAlpha
Plasmid#108651PurposeAlpha satellite repeats targeting Spy sgRNA under U6 promoterDepositorInsertsAlpha satellite repeats targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-2xNLS-mTurquoise2
Plasmid#118025PurposeAn AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoterDepositorInsert2xNLS-mTurquoise2
UseAAVTagsNLSPromoterhSyn1Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)
Plasmid#211861PurposeNuclear-localized E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-NLS-mCherry
Plasmid#193762PurposeDoxycycline-inducible (TetOn) mCherry with SV40 NLS, control constructDepositorInsertNLS-mCherry
UseLentiviralTagsmCherryMutationSV40 NLS fused to mCherryPromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NS3-NLS/VPR
Plasmid#112244PurposeEncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.DepositorInsertdCas9-NS3-NLS/VPR
TagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-SV40
Plasmid#117805PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xFNLS-P2A-Puro
Plasmid#110872PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS(3.7)
UseLentiviralTags3X FLAGMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
Tags6xHis and NLSExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mcGAS-LL/RK-NLS/D307A
Plasmid#185459PurposeInducible nuclear cGAS with D307A mutationDepositorInsertcGAS
UseLentiviralTagsFLAG and SV40 NLSExpressionMammalianMutationL157R, L159K, D307AAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET-B-evoSpCas9-NLS-6xHis
Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NLS
Plasmid#138214PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.DepositorInsertsACt-mCherry-NLS (Adcy10 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…PromoterEF1sAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianPromoterTRE-CMVAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LacI-NLS-VP16
Plasmid#103836PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInsertEGFP-LacI-NLS-VP16
ExpressionMammalianPromoterSV2Available SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tagRFP-NLS-HA-Bxb1
Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
TagsHA, SV40 NLS, and tagRFPExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
TagsHA, NLS(Makkerh)ExpressionMammalianAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS
Plasmid#124705PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLSDepositorInsertMCP_ADAR2 DD (E488Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:GFPNLS-SV40pA
Plasmid#155123PurposeTol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:GFPNLS
UseZebrafish expressionPromoterQUASM1-E1bAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
ExpressionBacterialAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-PGK-Puro
Plasmid#110844PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E5-dTom-nlsdTom
Plasmid#135640PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E5 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS
Plasmid#124626PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLSDepositorInsertMCP_ADAR1 DD (E1008Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE1008Q hyperactive mutantPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorInsertNLS-Smad2 (SMAD2 Human)
TagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
864 pSG5L HA NLS PTEN
Plasmid#10933DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pINO4-NLS-Pfv-Sapphire-HO
Plasmid#203660PurposeYeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.DepositorArticleInsertPfV
UseCRISPRTagsSapphireAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
nLYN267-gs10-NLS-VPR
Plasmid#188265PurposePlasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnLYN267 (LYN Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-NLS-BirA-2A-mCherry
Plasmid#127781PurposeBirA construct in a PCI nls backbone driving mCherry activityDepositorInsertBirA
UseUnspecifiedAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-NLS-AcrIIA4
Plasmid#113038PurposeAAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LAP2-YFP-NLS
Plasmid#25847DepositorInsertLAP2-YFP-NLS
TagsHis and NLSExpressionMammalianAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS
Plasmid#129530PurposeSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3NmeDepositorInsertscodon-optimized AcrIIC3Nme
sgRNA_Rosa26
UseAAVTagsFLAG/NLSPromoterU6 and cytomegalovirus-enhancer chicken β-actin p…Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only