Skip to main content
Addgene
Showing: 1051 - 1100 of 19141 results

  4. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

    Enlarge

  13. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid
    #50461
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    EF1a
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50461-plasmid-map-sequence-id-182308

    Enlarge

  19. L1-neo-TET

    Plasmid
    #51284
    Purpose
    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    L1RE1 (L1RE1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51284-plasmid-map-sequence-id-78366

    Enlarge

  21. pTriEx4-Cdc42-2G

    Plasmid
    #68814
    Purpose
    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
    Depositor
    Olivier Pertz
    Article
    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
    Insert
    Cdc42 activation reporter
    Use
    Tags
    His and Myc
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    68814-plasmid-map-sequence-id-120972

    Enlarge

  25. pUDP004

    Plasmid
    #101165
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101165-plasmid-map-sequence-id-199321

    Enlarge

  36. TFORF3549

    Plasmid
    #145025
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145025-plasmid-map-sequence-id-419572

    Enlarge

  40. pICH41331::pU6cm-pegR::EURb7

    Plasmid
    #227695
    Purpose
    Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
    Depositor
    Jae-Yean Kim
    Article
    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41
    Insert
    Altered epegRNA backbone with cloning sites for gRNA and RTT.
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    U6 composite
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227695-plasmid-map-sequence-id-452432

    Enlarge

  42. FUW mCherry-GFP-LC3

    Plasmid
    #110060
    Purpose
    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)
    Depositor
    Anne Brunet
    Article
    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283
    Insert
    mCherry-GFP-LC3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110060-plasmid-map-sequence-id-438935

    Enlarge

  47. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

    Enlarge

Showing: 1051 - 1100 of 19141 results