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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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161,152 results

Showing: 11151 - 11200 of 161152 results

  8. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

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  13. pLJM1 EGFP-Tau

    Plasmid
    #108868
    Purpose
    Expresses EGFP linked to human Tau
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    human four-repeat tau lacking the N-terminal sequ…
    Promoter
    CMV
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108868-plasmid-map-sequence-id-237064

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  25. hNFAT2-409:TALE:VP64:KRɸ

    Plasmid
    #160661
    Purpose
    Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.
    Depositor
    Roman Jerala
    Article
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Insert
    hNFAT2-409:TALE:VP64:KRɸ
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    160661-plasmid-map-sequence-id-338890

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  26. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Article
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    191015-plasmid-map-sequence-id-382132

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  29. ADRA1A-Tango

    Plasmid
    #66213
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA1A (ADRA1A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66213-plasmid-map-sequence-id-369270

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  35. VinTS-tCRMod-GGSGGS9

    Plasmid
    #111765
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111765-plasmid-map-sequence-id-214720

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  46. pMo130

    Plasmid
    #27388
    Purpose
    Burkholderia allelic exchange suicide vector pMo130, ref EU862243
    Depositor
    Martin Voskuil
    Article
    Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.
    Type
    Empty backbone
    Use
    Burkholderia allelic exchange suicide vector pmo1…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    27388-plasmid-map-sequence-id-455988

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  47. FPR1-Tango

    Plasmid
    #66283
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    FPR1 (FPR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. AAK1

    Plasmid
    #73244
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AAK1 (AAK1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 47-307
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 11151 - 11200 of 161152 results