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pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Talin1 head-mCherry
Plasmid#212005PurposeExpression of mouse talin1 head (1-490)-mCherry in mammalian cellsDepositorInsertMouse talin1 head domain (1-490) (Tln1 Mouse)
UseTagsmCherry fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF0
Plasmid#200380PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF1
Plasmid#200381PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyTag
Plasmid#59548PurposepET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramersDepositorInsertDead Streptavidin-SpyTag
UseTagsSpyTagExpressionBacterialMutationThe construct lacks final K of SpyTagPromoterT7Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIAL1
Plasmid#207669PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1DepositorInsertTIAL1 (TIAL1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-sfGFP-3xP3-RFP
Plasmid#127557PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(K42M)
Plasmid#165440PurposeExpresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)
UseTagsmEGFP(A206K)ExpressionMammalianMutationK42M: Kinase dead mutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HisB TO rFez1
Plasmid#17385DepositorInsertFEZ1 (Fez1 Rat)
UseTagshis and mycExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-DsRed-Express2
Plasmid#111251PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5B-CMV-GFP
Plasmid#83257PurposeAdenoviral expression of STAT5B with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox18
Plasmid#50782Purposeretroviral expression of mouse Sox18DepositorInsertSox18 (Sox18 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP
Plasmid#120831PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP
UseTagseYFP-V5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal80-3xP3-RFP
Plasmid#127562PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS3
Plasmid#120822PurposeEctopic expression of ZIKV proteinDepositorInsertNS3 ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
shCDK4/6(2)
Plasmid#73555Purposevector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)DepositorInsertshRNA against CDK4 + shRNA against CDK6
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKI WT
Plasmid#118300PurposeExpression of PKIaDepositorInsertPKI wt
UseTagsFlag and GFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR6
Plasmid#118997PurposeTargeting vector for human AAVS1 with TLR6 reporter insertDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDragon-DTA
Plasmid#155017PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activityDepositorInsertDTA
UseMouse TargetingTagsExpressionMutationtox176PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFlag-CMV7-Erk2_KR
Plasmid#39224DepositorInsertErk2(K52R) (Mapk1 Rat)
UseTags3xFLAGExpressionMammalianMutationK52RPromoterCMVAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCaRNA3-eIFiso4E120(+)
Plasmid#86092PurposeThis vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.DepositorInserteIFiso4E
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-His RTN3A1
Plasmid#31087DepositorInsertreticulon 3 (RTN3 Human)
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterATG8Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 S186D
Plasmid#16235DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationSer186 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
SB-SRT-Puro
Plasmid#154888PurposeSleeping Beauty self-reporting transposon with puromycin reporterDepositorInsertSleeping beauty SRT
UseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS2B
Plasmid#120819PurposeEctopic expression of ZIKV proteinDepositorInsertNS2B ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 T3550A/S3556A
Plasmid#199574PurposeExpresses FLAG-tagged mutated SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationT3550A and S3556APromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT-SEP
Plasmid#187653PurposeContains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.DepositorInsertHaloTag-SEP donor
UseAAV and CRISPRTagsN/AExpressionMutationPromoterN/AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
Plasmid#189795PurposeExpress GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
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p3E-V5-mScarlet-I_SV40pA (JDW 968)
Plasmid#224529PurposeA Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyADepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mVenus
Plasmid#137793PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Axin2 probe
Plasmid#21277DepositorInsertAxin2 (Axin2 Mouse)
UseFor riboprobe synthesisTagsExpressionMutationcDNA bp 1774 to 2787PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Mllt3
Plasmid#131604Purposeexpression of mouse MIIt3 in mammalian cellsDepositorInsertMllt3 (Mllt3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SUFU_Sufu
Plasmid#178488PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSUFU_Sufu (SUFU Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-PSM-Rb
Plasmid#37106DepositorInsertRb (RB1 Human)
UseRetroviralTagsExpressionMammalianMutationaa 379-928 only; large pocket of human Rb with aa…PromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG MSCV
Plasmid#8515DepositorInsertHOXA9 (Hoxa9 Mouse)
UseRetroviralTagsFLAG (DYKDDDDK) and IRES-GFPExpressionMammalianMutationmakes GFP from an IRES driving translation from a…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD077
Plasmid#136473PurposeSSB-mTur2 IDL fusion expression plasmidDepositorInsertSSB-mTur2 (ssb E. coli)
UseTagsmTurquoise2ExpressionBacterialMutationmTurquoise2 inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS_FLPER(T2)
Plasmid#31303DepositorInsertFLP-ER ligand binding domain (ESR1 Budding Yeast, Human)
UseTagsExpressionMammalianMutationfull length FLP linked to ligand binding domain o…PromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
3xmNeonGreen-3xrGBD
Plasmid#176101PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT26
Plasmid#170914PurposeGoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpEstradiol_InducibleAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only
