165,343 results

1. AAV-Camk2-NES-Twitch-GR

   Plasmid

   #173044

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Insert

   NES-Twitch-GR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamK2

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIX403-ccdB-G418

   Plasmid

   #158561

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG5-HA-p300

   Plasmid

   #89094

   Purpose

   mammalian expression of full-length human p300 in pSG5

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-NSP1SA11

   Plasmid

   #89168

   Purpose

   Express Rotavirus SA11 NSP1 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP1
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLXSN16E6E7

   Plasmid

   #52394

   Purpose

   The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

   Depositor

   Denise Galloway

   Article

   Halbert et al J Virol. 1991 Jan;65(1):473-8.

   Insert

   HPV16 E6E7
   

   Use

   Retroviral

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PBCAG-eGFP

   Plasmid

   #40973

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Inserts

   PiggyBac transposon 3' terminal repeats
   PiggyBac 5' terminal repeats
   

   Expression

   Mammalian

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. 768 pME mApple-CAAX

    Plasmid

    #195961

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple-CAAX (membrane)
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hsyn-NE2m

    Plasmid

    #208686

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-TRIM28

    Plasmid

    #65397

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

    Plasmid

    #209727

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGIG

    Plasmid

    #11159

    Purpose

    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. 764 pME nls-mApple

    Plasmid

    #195962

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. 765 pME nls mCerulean

    Plasmid

    #195958

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-ATG2C(VPS13C)

    Plasmid

    #232868

    Purpose

    Expresses the ATG2C domain of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the ATG2C domain of VPS13C (VPS13C Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 769 pME mCerulean-CAAX

    Plasmid

    #195957

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean-CAAX (membrane)
    

    Use

    Other

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFlag-CMV1-hMD2

    Plasmid

    #13028

    Depositor

    Doug Golenbock

    Insert

    MD2 (LY96 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Mutation

    none

    Promoter

    SP6

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-EGFP-RhoA-T19N

    Plasmid

    #12967

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA DN (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T19N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBA707

    Plasmid

    #186238

    Purpose

    KanR-BBR1 entry vector for HR donor

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-28a mScarlet

    Plasmid

    #178543

    Purpose

    The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mScarlet
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEYFP-N3-EPAC1

    Plasmid

    #113110

    Purpose

    Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

    Depositor

    Xiaodong Cheng

    Article

    Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

    Insert

    RapGEF3 (RAPGEF3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    PCMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCK054 pDEST exorh:mCerulean

    Plasmid

    #195985

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Alpha-Synuclein-His

    Plasmid

    #186802

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Alpha-Synuclein-His (SNCA Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDsRed-attP

    Plasmid

    #51019

    Purpose

    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Inserts

    attP
    LoxP-3xP3-DsRed-LoxP
    

    Use

    High copy, amp resistance

    Promoter

    3xP3 and None

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge