162,717 results

1. pAAV-CAG-mCardinal

   Plasmid

   #197195

   Purpose

   Expresses the protein of mCardinal in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mCardinal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3998 pGEX HHR23A

   Plasmid

   #10864

   Depositor

   Peter Howley

   Article

   Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

   Insert

   HHR23A (RAD23A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pXW109Hg(RS-RinA-E11)2

   Plasmid

   #123151

   Purpose

   Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, PmerT, PhrpLE, PrinA

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3puro-EGFP-GAPDH

   Plasmid

   #225475

   Purpose

   Express mEGFP-fusion protein of GAPDH

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   GAPDH (GAPDH Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11a_3xFlag-nsp14 (SARS-CoV-2)

   Plasmid

   #169159

   Purpose

   To express SARS-CoV-2 nsp14 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tubulin-LSSmGFP

   Plasmid

   #186535

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Tubulin

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPR77-Tango

   Plasmid

   #66373

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR77 (C5AR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. TFORF1569

   Plasmid

   #141841

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF680 (ZNF680 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GLE1

   Plasmid

   #155625

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GLE1 (GLE1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HMGA2+GFP

    Plasmid

    #25405

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    polyadenylation signal lacking

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCI-Myc3-HPS4

    Plasmid

    #133931

    Purpose

    Expresses 3xMyc-HPS4 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

    Insert

    HPS4 (HPS4 Human)
    

    Use

    Tags

    3xMyc tag

    Expression

    Mammalian

    Mutation

    Glutamic acid 229 to Glycine; Valine 552 to Methi…

    Promoter

    CMV I.E.

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1512

    Plasmid

    #144366

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX3-2 (NKX3-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-BOS-T7-CA150

    Plasmid

    #21924

    Depositor

    Mariano Garcia-Blanco

    Article

    Sune et al Mol Cell Biol. 1999 Jul . 19(7):4719-28.

    Insert

    CA150 (TCERG1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

14. C1ORF43-3xFLAG

    Plasmid

    #130880

    Purpose

    Expresses human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF0677

    Plasmid

    #142192

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM16 (PRDM16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE myc-Raptor S722A/S792A

    Plasmid

    #18117

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S722A, S792A

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pMpGWB326

    Plasmid

    #68654

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM60-Ptb

    Plasmid

    #38245

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Ptb (Ptbp1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP1159

    Plasmid

    #119250

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HMGA2(3'UTR DEL)+GFP

    Plasmid

    #25406

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3" UTR is absent

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

22. TFORF3023

    Plasmid

    #144499

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROD6 (NEUROD6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR221-Hyg

    Plasmid

    #48994

    Purpose

    Gateway adapted transfer of Hygromycin resistance gene

    Depositor

    Rick Tarleton

    Article

    Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

    Insert

    hygromycin B phosphotransferase
    

    Use

    Gateway attb1 & attb2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. HMGA2 shRNA

    Plasmid

    #25408

    Depositor

    Sean Morrison

    Article

    Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

    Insert

    high mobility group AT-Hook 2 (Hmga2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

25. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

    Plasmid

    #207123

    Purpose

    Lentiviral vector to express human METTL3

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    hMETTL3 (METTL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

    Plasmid

    #121448

    Purpose

    To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

    Depositor

    Brian Chen

    Article

    Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

    Insert

    RFPnols-PQR-ZeocinR (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF1800

    Plasmid

    #143546

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    USF2 (USF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF2884

    Plasmid

    #143654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FIGLA (FIGLA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kohinoor-Vimentin/pcDNA3

    Plasmid

    #67772

    Purpose

    Vimentin-targeted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor-Vimentin
    

    Use

    Tags

    Vimentin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

31. BMPR2

    Plasmid

    #38972

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pCR1276

    Plasmid

    #111115

    Purpose

    Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51C gRNA (RAD51C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GART_pCSdest

    Plasmid

    #53859

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    GART (GART Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

34. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Promoter

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAD100-CHIP-myc-His

    Plasmid

    #236054

    Purpose

    Mammalian expression of human CHIP with a C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP (STUB1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RNF43-LgBiT, BiBiT-Ready Vector

    Plasmid

    #237082

    Purpose

    Express RNF43-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    RNF43 (RNF43 Human)
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. HiBiT-TDP1 Fusion Vector

    Plasmid

    #236891

    Purpose

    Express HiBiT-TDP1 in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    TDP1 (TDP1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pNL [NlucP/STAT5-RE/Hygro] Vector

    Plasmid

    #236819

    Purpose

    Express STAT5 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    STAT5 (STAT5A Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Expression

    Mutation

    None

    Promoter

    minP

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. PiggyBac-TDP-43deltaNLS-V5-APEX

    Plasmid

    #229081

    Purpose

    Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

    Depositor

    Eran Hornstein

    Insert

    TDP-43deltaNLS (TARDBP Human)
    

    Use

    Tags

    APEX, BFP, and V5

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, K95A, K97A and R98A for mutatio…

    Promoter

    TRE3G

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EcoHIV-mScarlet

    Plasmid

    #235598

    Purpose

    EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

    Depositor

    Michael Shtutman

    Article

    Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

    Insert

    NL4-3 EcoHIV mScarlet
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge