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167,150 results

Showing: 1101 - 1150 of 167150 results

  2. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Tags
    NO
    Expression
    Bacterial
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  5. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available Since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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  9. C3AR1-DuET

    Plasmid
    #213191
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C3AR1 (C3AR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213191-plasmid-map-sequence-id-430756

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  12. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  17. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

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  37. pSBbi-BN

    Plasmid
    #60518
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60518-plasmid-map-sequence-id-97528

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  38. LentiV_Neo_SIK3_CR_K95M

    Plasmid
    #108106
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108106-plasmid-map-sequence-id-207840

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  44. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid
    #122200
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9
    TagRFP-T
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122200-plasmid-map-sequence-id-236123

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  47. lucMAPT-30D

    Plasmid
    #214672
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214672-plasmid-map-sequence-id-424908

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  48. lucMAPT-30U

    Plasmid
    #214673
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214673-plasmid-map-sequence-id-424933

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  49. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid
    #230043
    Purpose
    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.
    Depositor
    Sumeet Pal Singh
    Article
    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:
    Insert
    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    fabp10a
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    230043-plasmid-map-sequence-id-461059

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  50. mEmerald-Coronin1B-N-10

    Plasmid
    #54050
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Coronin1B (CORO1B Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1101 - 1150 of 167150 results