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162,651 results

Showing: 11501 - 11550 of 162651 results

  3. pBAD-pHScarlet

    Plasmid
    #191258
    Purpose
    Bacterial expression plasmids for pH sensitive fluorescent protein pHScarlet
    Depositor
    Robert Campbell
    Insert
    pHScarlet
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191258-plasmid-map-sequence-id-374867

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  5. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Samson Jacob
    Article
    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    194250-plasmid-map-sequence-id-391338

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  7. TFORF1218

    Plasmid
    #142157
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRRX1 (PRRX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142157-plasmid-map-sequence-id-391449

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  8. pLenti6-MK1-EHMT2-V5

    Plasmid
    #31113
    Depositor
    Bernard Futscher
    Insert
    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Stop codon mutated to Ala
    Promoter
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    31113-plasmid-map-sequence-id-364693

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  10. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    Ana Egana, New England Biolabs
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101137-plasmid-map-sequence-id-194162

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  12. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217345
    Purpose
    crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
    crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
    crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
    crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217345-plasmid-map-sequence-id-426760

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  18. pSLIK-Hygro

    Plasmid
    #25737
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Academic Institutions and Nonprofits only
    25737-plasmid-map-sequence-id-202030

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  19. mTagRFP-T-Lifeact-7

    Plasmid
    #54586
    Purpose
    Localization: Actin, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    LifeAct -mTagRFP-T
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54586-plasmid-map-sequence-id-176185

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  21. TFORF0318

    Plasmid
    #143392
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF117 (ZNF117 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143392-plasmid-map-sequence-id-397901

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  24. CAG-AausFP1-Z1

    Plasmid
    #135047
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135047-plasmid-map-sequence-id-271473

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  29. TFORF3139

    Plasmid
    #144615
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARG (PPARG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144615-plasmid-map-sequence-id-346845

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  41. pAAV hPGRN

    Plasmid
    #213682
    Purpose
    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human Progranulin (hPGRN) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and V5 tag (after signal peptide)
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213682-plasmid-map-sequence-id-455751

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  46. ColorfulCell

    Plasmid
    #62449
    Purpose
    MXS_chaining vector with 6 cassettes
    Depositor
    Pierre Neveu
    Article
    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do
    Inserts
    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    62449-plasmid-map-sequence-id-209509

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  47. pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156948
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NCAM1 (NCAM1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156948-plasmid-map-sequence-id-412906

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  49. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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Showing: 11501 - 11550 of 162651 results