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179,521 results

Showing: 9401 - 9440 of 179521 results

  5. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84240-plasmid-map-sequence-id-160812

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  6. Grp1-PH pEGFP-C1

    Plasmid
    #71378
    Purpose
    For analysis of PH domain localization in mammalian cells
    Depositor
    Mark Lemmon
    Publication
    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Grp1-PH (Cyth3 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin homology (PH) domain; aa 247-399
    Promoter
    CMV
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71378-plasmid-map-sequence-id-422685

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  8. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75024-plasmid-map-sequence-id-450679

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  12. CAG sHRPa-NRX

    Plasmid
    #73143
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-NRX
    Tags
    2x HA tag and Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73143-plasmid-map-sequence-id-134511

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  17. alpha catenin conformation sensor

    Plasmid
    #71709
    Purpose
    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71709-plasmid-map-sequence-id-142365

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  20. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Publication
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  24. pHBV-Luc

    Plasmid
    #71414
    Purpose
    To see the activity of HBV transcription using Luciferase assay
    Depositor
    Wang-Shick Ryu
    Publication
    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HBV core promoter, enhancer I, enhancer II and luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    HBV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71414-plasmid-map-sequence-id-132686

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  27. p5E-mfap4

    Plasmid
    #70052
    Purpose
    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
    Depositor
    David Tobin
    Publication
    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do
    Insert
    mfap4 promoter
    Use
    Gateway 5' element
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70052-plasmid-map-sequence-id-130081

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  29. GPR120-Tango

    Plasmid
    #66310
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR120 (FFAR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66310-plasmid-map-sequence-id-248122

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  39. TGFB1-bio-His

    Plasmid
    #52185
    Purpose
    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TGFB1 (TGFB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52185-plasmid-map-sequence-id-80549

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  40. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62204-plasmid-map-sequence-id-521112

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Showing: 9401 - 9440 of 179521 results