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161,805 results

Showing: 9401 - 9440 of 161805 results

  8. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

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  10. Rlucrarr3-Flash5

    Plasmid
    #74133
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3-TLR2-CFP

    Plasmid
    #13015
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13015-plasmid-map-sequence-id-471621

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  14. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

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  15. pET11b-MIF

    Plasmid
    #149446
    Purpose
    Expression of WT MIF in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149446-plasmid-map-sequence-id-311585

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  16. PSD-95-pTagRFP

    Plasmid
    #52671
    Purpose
    expression of TagRFP-tagged PSD-95
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Use
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52671-plasmid-map-sequence-id-272433

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  17. pRetroX-TRE3G_CBP-GFP

    Plasmid
    #221926
    Purpose
    Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction
    Depositor
    Daniel Bose
    Insert
    CBP (CREBBP Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    insertion of CBP-GFP
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221926-plasmid-map-sequence-id-437450

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  18. pENN.AAV.CAG.tdTomato.WPRE.SV40

    Plasmid
    #105554
    Purpose
    AAV expression of tdTomato from CAG promoter
    Depositor
    James M. Wilson
    Insert
    tdtomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105554-plasmid-map-sequence-id-202390

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  19. TetR in pENTR1A (559-1)

    Plasmid
    #22265
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor (tetR )
    Use
    Tags
    Expression
    Insect, Mammalian, Worm Exp…
    Mutation
    Promoter
    Available since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22265-plasmid-map-sequence-id-316914

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  22. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  28. pX551-miniCMV-SpCas9

    Plasmid
    #107031
    Purpose
    AAV plasmid expressing Cas9 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107031-plasmid-map-sequence-id-208545

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  31. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Promoter
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

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  33. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

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  35. FUGW-APOE-E3-T2A-mCherry

    Plasmid
    #190808
    Purpose
    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190808-plasmid-map-sequence-id-372853

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  37. pAAV-nEF-Coff/Fon-ChRmine-oScarlet

    Plasmid
    #137160
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Coff/Fon-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137160-plasmid-map-sequence-id-272778

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Showing: 9401 - 9440 of 161805 results