165,343 results

1. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Roger Tsien, Nathan Shaner, Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HRE:GFP-pGK:Cre

   Plasmid

   #68552

   Purpose

   Lentivirus expressing hypoxia-responsive GFP reporter and Cre

   Depositor

   Tyler Jacks

   Inserts

   Green Fluorescent Protein
   Cre
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   HRE and pGK

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPLICS Mt-ER- Po Short P2A

   Plasmid

   #164118

   Purpose

   Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Short P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Mutation

   H840A

   Promoter

   EF1a

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_118

   Plasmid

   #133459

   Purpose

   U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rtn4a-mNeonGreen

   Plasmid

   #169032

   Purpose

   Express human Rtn4a

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rtn4a (RTN4 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV-Cre pLKO.1

    Plasmid

    #25997

    Purpose

    Lentiviral expression of nls-Cre from the CMV promoter

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1.Gαs-LgB113

    Plasmid

    #134362

    Purpose

    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαs-LgB113 (GNAS Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid

    #66128

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Promoter

    VEGF -1000 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLminP_Luc2P_RE34

    Plasmid

    #90376

    Purpose

    MAPK - Elk1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    ELK1

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPLICSFAST-ER-MT-P2A

    Plasmid

    #214892

    Purpose

    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-MT-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPLICS Po-ER Long P2A

    Plasmid

    #164113

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-mEGFP-CREB (S133A)

    Plasmid

    #137002

    Purpose

    mEGFP-CREB donor for G-CREB with S133A mutation

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Changes Serine 133 into Alanine

    Promoter

    CMV

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Tet-O-FUW-Neurod1

    Plasmid

    #30129

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    NeuroD1 (Neurod1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pYIC

    Plasmid

    #18673

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Type

    Empty backbone

    Tags

    3Myc-EYFP-HA-His6 and ECFP-HA-His6

    Expression

    Mammalian

    Available Since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHCL151

    Plasmid

    #134455

    Purpose

    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

    Depositor

    Thomas Bernhardt

    Article

    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

    Insert

    linker-mScarlet-I for C-terminal fusion
    

    Expression

    Bacterial

    Promoter

    plac

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPB-EF1A-Puro-hTBK1-E696K-EGFP

    Plasmid

    #221554

    Purpose

    Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed glutamate 696 to lysine

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115219

    Purpose

    Expresses PspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PspCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Available Since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMP31-1

    Plasmid

    #45905

    Depositor

    Harmit Malik

    Article

    Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

    Insert

    mitochondrial antiviral signaling protein (MAVS Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBMN_HA-TBK1 (kinase dead - D135N)

    Plasmid

    #199206

    Purpose

    Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D135N

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. integrin beta6 EGFP-N3

    Plasmid

    #13593

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EBFP2-Mito-7

    Plasmid

    #55248

    Purpose

    Localization: Mitochondria, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Mito (COX8A Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189922

    Purpose

    AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e
    

    Use

    AAV

    Mutation

    SaCas9 D10A

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1.Gαi3-LgB91

    Plasmid

    #134359

    Purpose

    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi3-LgB91 (GNAI3 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. [#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

    Plasmid

    #233507

    Purpose

    Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    DsRed loxP eGFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE-PIK3CA-E542K

    Plasmid

    #116479

    Purpose

    Lentiviral expression of PIK3CA E542K

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    E542K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge