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165,255 results

Showing: 2501 - 2550 of 165255 results

  6. pLX302

    Plasmid
    #25896
    Purpose
    3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.
    Depositor
    David Root
    Article
    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 30, 2010
    Availability
    Academic Institutions and Nonprofits only
    25896-plasmid-map-sequence-id-364646

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  7. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    P45
    Use
    Synthetic Biology
    Available Since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  8. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Promoter
    Custom
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  14. MCP-NMS

    Plasmid
    #210705
    Purpose
    This plasmid express EF1a promoter driven MCP-NMS transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210705-plasmid-map-sequence-id-416249

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  23. pTIPI_mIGg2a_Anti-HA [12CA5]

    Plasmid
    #210656
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] mouse IgG2a heavy chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse IgG2a heavy chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210656-plasmid-map-sequence-id-416221

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  24. pTIPI_mK_Anti-HA [12CA5]

    Plasmid
    #210657
    Purpose
    Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] kappa light chain
    Use
    Affinity Reagent/ Antibody
    Tags
    Mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    210657-plasmid-map-sequence-id-416222

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  26. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  30. pBMN-I-GFP

    Plasmid
    #1736
    Purpose
    This retroviral vector has an IRES-GFP downstream of the cloning sites
    Depositor
    Garry Nolan
    Insert
    IRES-GFP
    Use
    Retroviral
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1736-plasmid-map-sequence-id-418359

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  33. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  38. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  43. pLEX_307

    Plasmid
    #41392
    Purpose
    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41392-plasmid-map-sequence-id-331248

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  44. pSLIK-Hygro

    Plasmid
    #25737
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    Aug. 13, 2010
    Availability
    Academic Institutions and Nonprofits only
    25737-plasmid-map-sequence-id-202030

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  48. pLenti-MetGFP

    Plasmid
    #37560
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    37560-plasmid-map-sequence-id-339875

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  50. pBluescript-minimal PB

    Plasmid
    #177151
    Purpose
    Minimal piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177151-plasmid-map-sequence-id-348859

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Showing: 2501 - 2550 of 165255 results