163,584 results

1. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-3FLAG-EEA1

   Plasmid

   #176491

   Purpose

   For lentiviral transduction of 3FLAG-EEA1

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-NLRC5

   Plasmid

   #37521

   Depositor

   Thomas Kufer

   Article

   Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

   Insert

   NLRC5 (NLRC5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Use

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-C3-hYAP1

   Plasmid

   #17843

   Depositor

   Marius Sudol

   Article

   Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD24-fis

   Plasmid

   #51557

   Purpose

   E. coli fis ORF cloned into pBAD24 expression vector

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   FIS (fis E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScarlet-N1

   Plasmid

   #128060

   Purpose

   Mammalian expression vector for C terminal mScarlet-I fusions

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TOMM20-mTurquoise-Rac1-G12V-deltaCaaX

    Plasmid

    #176124

    Purpose

    Constitutively active, mitochondrial localized Rac1

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF6-Lck-TurboID

    Plasmid

    #159433

    Purpose

    expresses Lck-TurboID in mammalian cells

    Depositor

    Arthur Salomon

    Article

    Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

    Inserts

    Lck (LCK Human)
    TurboID (BirA mutant)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    EF-1a

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ10530

    Plasmid

    #239266

    Purpose

    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    NES-dPspCas13b-EGFP-ABI
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag tag and V5 tag

    Expression

    Mammalian

    Mutation

    H133A and H1058A in PspCas13b

    Promoter

    EF1a

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-iCasp9

    Plasmid

    #199441

    Purpose

    Express inducible Caspase9 in mammalian cells

    Depositor

    Chao Tang

    Article

    Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

    Insert

    inducible Caspase9 (CASP9 Human)
    

    Use

    Lentiviral

    Tags

    DmrB and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRham-CALM1

    Plasmid

    #182500

    Purpose

    Bacterial expression of the Homo sapiens calmodulin, CALM1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    CALM1 (CALM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-Gsdmd-NT

    Plasmid

    #80951

    Purpose

    Expresses Gsdmd-NT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LifeAct-HaloTag

    Plasmid

    #176105

    Purpose

    F-actin marker

    Depositor

    Dorus Gadella

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIVTRup

    Plasmid

    #101362

    Purpose

    Highly efficient in vitro transcription. Empty backbone.

    Depositor

    Ángel Raya

    Insert

    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    

    Use

    For cloning a gene for in vitro transcription pur…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCaggs-mGold2s

    Plasmid

    #231763

    Purpose

    Expression of mGold2s in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Flag-HA-USP5

    Plasmid

    #22590

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP5 (USP5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX puro TRIM21-GFP

    Plasmid

    #116941

    Purpose

    Expression of TRIM21-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 - GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3/Flag-WTAP

    Plasmid

    #53741

    Purpose

    For expression of WTAP (Wilms tumor 1 associated protein) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Wilms tumor 1 associated protein (WTAP Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid

    #80922

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLVX-puro-cOVA-IRES-BFP

    Plasmid

    #135074

    Purpose

    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

    Depositor

    Maria Castro

    Insert

    cOVA-IRES-BFP (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge