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177,145 results

Showing: 2751 - 2800 of 177145 results

  1. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    20 Articles
    72266-plasmid-map-sequence-id-447561

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  3. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Publication
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    63891-plasmid-map-sequence-id-450336

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  18. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    139462-plasmid-map-sequence-id-308474

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  23. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid
    #112021
    Purpose
    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NYESO beta/alpha into TRAC HDRT
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112021-plasmid-map-sequence-id-215596

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  32. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    45945-plasmid-map-sequence-id-116251

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  44. FCK-Halo-GFP

    Plasmid
    #14750
    Purpose
    Lentiviral expression of Halo-GFP for neuronal inhibition
    Depositor
    Edward Boyden
    Publication
    Han et al PLoS One. 2007 Mar 21;2(3):e299.
    Insert
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N. pharaonis halorhodopsin is mammalian codon-opt…
    Promoter
    CaMKIIa
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    14750-plasmid-map-sequence-id-192940

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  48. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid
    #181751
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    181751-plasmid-map-sequence-id-353935

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  49. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    177938-plasmid-map-sequence-id-429078

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Showing: 2751 - 2800 of 177145 results