159,238 results

1. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Use

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA ZIP-GFP11

   Plasmid

   #182242

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP11

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   ZIP-GFP11
   

   Use

   Tags

   split-GFP11 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEM415-ldhL-mRFP1

   Plasmid

   #99842

   Purpose

   Expression of RFP in Lactobacillus

   Depositor

   Sujin Bao

   Article

   Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   ldhl

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTight-9-124-BclxL

   Plasmid

   #60857

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTight

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-3xNES-ABE7.10-NG

   Plasmid

   #181753

   Purpose

   MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE7.10-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRMC2

   Plasmid

   #68940

   Purpose

   tetracycline-inducible expression vector for Staphylococcus aureus

   Depositor

   Tim Foster

   Article

   Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

   Type

   Empty backbone

   Use

   Tet inducible e. coli/staphylococcus shuttle vect…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. BFP-KDEL

   Plasmid

   #49150

   Purpose

   visualization of the ER

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   BiP ER signal sequence (HSPA5 Human)
   

   Use

   Tags

   BFP and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p37-2iDMD-LR

    Plasmid

    #88892

    Purpose

    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dystrophin (DMD Human)
    luciferase
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EGFP-Cre

    Plasmid

    #86805

    Purpose

    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter

    Depositor

    Amy Lasek

    Article

    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    EGFP and nuclear localization signal: PKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-IRES-Blast

    Plasmid

    #85133

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8r

    Plasmid

    #208294

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Fos-CreERT2

    Plasmid

    #194643

    Purpose

    Expresses Cre-ERT2 under the control of c-Fos promoter

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    Fos-CreERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFX-EGFP-EEA1

    Plasmid

    #174451

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Use

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ubiquitin C

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-Malibu-mScarlet

    Plasmid

    #229554

    Purpose

    Fluorescent reporter for malonyl-CoA

    Depositor

    Danielle Schmitt

    Article

    Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

    Insert

    Malibu-mScarlet
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

    Plasmid

    #173899

    Purpose

    The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

    Depositor

    Jason W Chin

    Article

    Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

    Inserts

    ThrRS(v1.0)
    tRNA(v2.0)CUA
    pduX
    EF-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hChATp-tdTomato

    Plasmid

    #197564

    Purpose

    Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

    Depositor

    Casey Maguire

    Article

    Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human ChAT promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pgRNA-bacteria

    Plasmid

    #44251

    Purpose

    Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDGE687

    Plasmid

    #167299

    Purpose

    Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. GSK3 beta pGEX

    Plasmid

    #15898

    Depositor

    Jim Woodgett

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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