4,134 results

1. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LeGO-C2

   Plasmid

   #27339

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSL2680

   Plasmid

   #85581

   Purpose

   Replicating base vector for constructing cpf1/CRISPR editing plasmids

   Depositor

   Himadri Pakrasi

   Article

   Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO-puro FLAG SREBP2

   Plasmid

   #32018

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV PIG (Puro IRES GFP empty vector)

   Plasmid

   #21654

   Purpose

   Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. ClopHensorN

   Plasmid

   #50758

   Purpose

   Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

   Depositor

   Colin Akerman

   Article

   Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

   Insert

   ClopHensorN
   

   Use

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   CAG

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-DDB1

   Plasmid

   #19918

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IMS-APEX2

    Plasmid

    #79058

    Purpose

    Expresses APEX in the mitochondrial intermembrane space.

    Depositor

    Alice Ting

    Article

    Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

    Insert

    APEX
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6-MK1-EHMT2-V5

    Plasmid

    #31113

    Depositor

    Bernard Futscher

    Insert

    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Stop codon mutated to Ala

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-HA-PSMD14

    Plasmid

    #22557

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    PSMD14 (PSMD14 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-ack

    Plasmid

    #62654

    Purpose

    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    exo, beta, gam, sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pALB-GFP

    Plasmid

    #55759

    Purpose

    Fluorescent marker for hepatocytic lineage

    Depositor

    Snorri Thorgeirsson

    Article

    Heo et al Hepatology. 2006 Dec;44(6):1478-86.

    Insert

    albumin enhancer/promoter (Alb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Albumin Enhancer/Promoter

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    tandem EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-hPIASx alpha

    Plasmid

    #15209

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx alpha (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    synthetic U6 promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-ClopHensor

    Plasmid

    #25938

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    ClopHensor
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DsRed-rab7 WT

    Plasmid

    #12661

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEP4 E02S CK2M EN2L

    Plasmid

    #20924

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Nathaniel Heintz, Alexander Nectow, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73037

    Purpose

    Express Nluc and copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-Neo-Bam p53 wt

    Plasmid

    #16434

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.cTNT.iCre

    Plasmid

    #69916

    Purpose

    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

    Has Service

    AAV9

    Insert

    improved cre (iCre) recombinase and tdTomato
    

    Use

    AAV

    Tags

    The iCre coding frame and tdTomato coding frame i…

    Expression

    Mutation

    Promoter

    Chicken cardiac troponin T

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid

    #60365

    Purpose

    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMVHA hEZH2

    Plasmid

    #24230

    Purpose

    Mammalian expression of human EZH2 with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PSD-95-pTagRFP

    Plasmid

    #52671

    Purpose

    expression of TagRFP-tagged PSD-95

    Depositor

    Johannes Hell

    Insert

    PSD-95 (Dlg4 Rat)
    

    Use

    Tags

    tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Str-Golgin84_VSVG-SBP-EGFP

    Plasmid

    #65305

    Purpose

    synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBI-MCS-EGFP

    Plasmid

    #16542

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.3_eGFP

    Plasmid

    #26822

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG_smFP Myc

    Plasmid

    #59757

    Purpose

    encodes multiple copies of an epitope tag Myc

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _Myc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP VAMP2

    Plasmid

    #42308

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

    Insert

    VAMP 2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge