4,114 results
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hTLR2 flag
Plasmid#13082DepositorInsertTLR2 (TLR2 Human)
UseTagsFlagExpressionMammalianMutationAmino acids 1-16 have been replaced with mammalia…PromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A T22N
Plasmid#28048DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationT22NPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH1-FLAG
Plasmid#66802PurposeTet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625PurposeTet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS6
Plasmid#55752PurposeProduces T7 RNA Polymerase.DepositorInsertT7 RNA Polymerase
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
myrAkt delta4-129
Plasmid#10841DepositorInsertAkt (AKT1 Human)
UseTagsHA and myrExpressionMammalianMutationDeletion of PH domain (aa4-129)PromoterSV40Available sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta1
Plasmid#16042DepositorInsertbeta1 (ITGB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
Plasmid#141370PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Vinculin-venus
Plasmid#27300DepositorInsertVinculin (VCL Chicken)
UseTagsVenus A206KExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-cyclinB1-mcherry (1958)
Plasmid#26063DepositorInsertCyclin B1 (CCNB1 Human)
UseTagsmcherryExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor
Plasmid#26633DepositorInsertraptor (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAL4
Plasmid#24344DepositorInsertGAL4
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-BlaM-Vpr
Plasmid#21950DepositorInsertBlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA EYFP
Plasmid#10825DepositorInsertEYFP
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA PKB T308D S473D pcDNA3
Plasmid#14751DepositorInsertPKB DD (AKT1 Bovine)
UseTagsHAExpressionMammalianMutationT308D S473D. Constitutively active mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAVS1-NC-CRISPRi (Gen3)
Plasmid#73499PurposeConstitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locusDepositorInsertdCas9-KRAB
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-modified
Plasmid#84477Purposeguide RNA expression lentiviral constructDepositorInsertguide RNA
UseLentiviralTagsExpressionMammalianMutationPromotermU6Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorInsertintegrin beta 3 (ITGB3 Human)
UseTagsHis and MycExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pDESTmycDDX17
Plasmid#19876DepositorInsertDEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP C99
Plasmid#30146DepositorInsertAPP C99 (APP Human)
UseTagsExpressionMammalianMutationC-terminal from BACE site of APPPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+) Drp1
Plasmid#34706DepositorInsertDrp1 (Dnm1l Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBB541
Plasmid#27394DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY109 (lenti-LbCpf1)
Plasmid#84740PurposeLenti virus delivery of LbCpf1 and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseLentiviralTags3xHA, NLS, and P2AExpressionMutationPromoterEFSAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-RFP-ATG9A
Plasmid#60609PurposeExpresses RFP-hATG9A in mammalian cellsDepositorInsertHomo sapiens autophagy related 9A (PPARG Human)
UseRetroviralTagsRFPExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Centrin2 (Nigg UK185)
Plasmid#41147DepositorInsertCentrin-2 (CETN2 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic EKAR (EGFP-mRFP)
Plasmid#18680DepositorInsertERK Activity Reporter
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLX302_FOXA1-V5
Plasmid#70090PurposeExpression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vectorDepositorInsertFOXA1 (FOXA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSlow-FT-N1
Plasmid#31912DepositorInsertSlow-FT
UseTagsExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pX330a dCas9-LSD1
Plasmid#92362PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.DepositorInsertdCas9-LSD1
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-N1
Plasmid#11909DepositorHas ServiceCloning Grade DNAInsertpPAGFP-N1
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
mApple-pBAD
Plasmid#54536PurposeLocalization: Protein Expression Vector , Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTags6xHis and mAppleExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-delta30-TFEB
Plasmid#44445DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationDeleted amino acids 1-30; H32DPromoterAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FLT3
Plasmid#23895DepositorInsertFLT3 (FLT3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Zeo (637-7)
Plasmid#17449Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, ZeoDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
V245 pCEP-4HA B56alpha
Plasmid#14532DepositorInsertPPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
UseTags4 HAExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSKI015
Plasmid#11570DepositorInsertCaMV 35S enhancers
UseActivation taggingTagsExpressionPlantMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SC35-cMyc
Plasmid#44721DepositorInsertSC35 (SRSF2 Human)
UseTagshis and mycExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV PIG (Puro IRES GFP)
Plasmid#18751DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-bleo IGF-1R
Plasmid#11212DepositorInsertIGF-1R (IGF1R Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only
