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20,198 results

Showing: 2561 - 2600 of 20198 results

  3. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Ulrik Gether
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177328-plasmid-map-sequence-id-349583

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  5. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  9. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  10. AAV.TBG.PI.Cre.rBG

    Plasmid
    #107787
    Purpose
    AAV vector expressing Cre from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    107787-plasmid-map-sequence-id-259375

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  15. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

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  16. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  18. mCherry-Rab5a-7

    Plasmid
    #55126
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55126-plasmid-map-sequence-id-133168

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  19. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80899-plasmid-map-sequence-id-181185

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  22. mEGFP-N1

    Plasmid
    #54767
    Purpose
    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54767-plasmid-map-sequence-id-204493

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  25. pLKO.3G

    Plasmid
    #14748
    Depositor
    Christophe Benoist, Diane Mathis
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  34. pCAG HRP-TM

    Plasmid
    #44441
    Depositor
    Alice Ting
    Article
    Rhee et al Science. 2013 Jan 31.
    Insert
    Horseradish peroxidase fused to the transmembrane domain of PDGFR
    Tags
    HA tag and myc tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44441-plasmid-map-sequence-id-337239

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  40. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

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Showing: 2561 - 2600 of 20198 results