We narrowed to 15,371 results for: genome

1. pJC146.4-spp-13

   Plasmid

   #26498

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Insert

   spp-13
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pJC99.1-npy-9

   Plasmid

   #26465

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Insert

   npy-9
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pJC98.1-npy-4

   Plasmid

   #26466

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Insert

   npy-4
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pSKB3.MPN625

   Plasmid

   #11430

   Depositor

   Sung-Hou Kim

   Article

   Choi et al J Struct Funct Genomics. 2003 . 4(1):31

   Insert

   peroxiredoxin (MPN625 M. pneumoniae)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pLIC.B4.BSU37660

   Plasmid

   #11535

   Depositor

   Sung-Hou Kim

   Article

   Xu et al J Struct Funct Genomics. 2005 Nov 9. ():

   Insert

   phosphotransacetylase
   

   Use

   Tags

   HisTag-MBP, TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

6. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLCHKOv3-mCherry-intergenic-As

   Plasmid

   #209037

   Purpose

   Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAav-MDM2-PQS2-3xHA

   Plasmid

   #84886

   Purpose

   rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Inserts

   MDM2 genomic sequence HR1 (MDM2 Human)
   MDM2 genomic insert HR2 (MDM2 Human)
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Expression

   Mutation

   Promoter

   no

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCHKOv3-mClover-intergenic-As

    Plasmid

    #209036

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLCHKOv3-mClover-intergenic_2-As

    Plasmid

    #218814

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCHKOv3-mClover-intergenic_3-As

    Plasmid

    #218815

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCHKOv3-mClover-intergenic_4-As

    Plasmid

    #218816

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCR2.1-myco

    Plasmid

    #36870

    Depositor

    Mario Chin

    Insert

    myco
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF-APEX2-NLS

    Plasmid

    #140281

    Purpose

    Expression of APEX2-NLS

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    APEX2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas

    Plasmid

    #62225

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgR

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Has Service

    Cloning Grade DNA

    Insert

    cas9
    

    Use

    CRISPR; Lambda red recombinase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    native cas9 promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

    Plasmid

    #176271

    Purpose

    Expression of CasMINI-V3.1 in mammalian cells.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI-V3.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4xMS2-hTR

    Plasmid

    #154940

    Purpose

    4xMS2 stem-loop-hTR-500 bp genomic sequence

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    4xMS2 stem-loop-hTR-500 bp genomic sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. psPAX2-D64V

    Plasmid

    #63586

    Purpose

    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

    Depositor

    David Rawlings, Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Packaging plasmid for integrase deficient lentiviral vectors
    

    Use

    Integrase deficient lentiviral packaging vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gRNA Cloning Vector Bbs I ver. 2

    Plasmid

    #85586

    Purpose

    An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid

    #80921

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW57-MCS1-2A-MCS2

    Plasmid

    #71782

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-minP:luc2

    Plasmid

    #138354

    Purpose

    Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

32. pJH136

    Plasmid

    #47554

    Purpose

    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

    Insert

    URA3
    

    Use

    Pcr template

    Tags

    Expression

    Mutation

    Promoter

    Native URA3 promoter, -243 to -1.

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTXB1-Tn5

    Plasmid

    #60240

    Purpose

    IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Rickard Sandberg

    Article

    Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

    Insert

    tnpA from pBam1
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7 promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRANS_232

    Plasmid

    #91121

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBECAb-apr

    Plasmid

    #122001

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFETCh_Donor (EMM0021)

    Plasmid

    #63934

    Purpose

    3X Flag with P2A-Neo for 3' tagging of endogenous proteins

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Glycine-Serine-3XFlag-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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43. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCnRed

    Plasmid

    #232908

    Purpose

    Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    gam (gam Synthetic, phage Lambda)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pbad-rha

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBAM1

    Plasmid

    #60487

    Purpose

    This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Use

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLC-HA-Nedd8-P2A-Hygro

    Plasmid

    #124306

    Purpose

    Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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