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177,146 results

Showing: 3801 - 3850 of 177146 results

  1. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51087
    Purpose
    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    CaMKIIa 1.3 kb
    Available since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    51087-plasmid-map-sequence-id-77369

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  2. M-NMn-VP64

    Plasmid
    #48676
    Purpose
    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    48676-plasmid-map-sequence-id-71851

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  3. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    10 Articles
    47024-plasmid-map-sequence-id-67042

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  11. pCW-LIC

    Plasmid
    #26098
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Three consecutive Taq promoters, 2 active
    Expression
    Bacterial
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    12 Articles
    26098-plasmid-map-sequence-id-251071

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  26. mTLR4

    Plasmid
    #13085
    Depositor
    Ruslan Medzhitov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TLR4 (Tlr4 Mouse)
    Expression
    Mammalian
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    13085-plasmid-map-sequence-id-142564

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  31. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  32. pCMV-15F11-HA-Halo

    Plasmid
    #129592
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    129592-plasmid-map-sequence-id-254268

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  35. GFP-hPIKfyve

    Plasmid
    #121148
    Purpose
    Expresses GFP-tagged human PIKfyve in mammalian cells.
    Depositor
    Geert van den Bogaart
    Publication
    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    121148-plasmid-map-sequence-id-237778

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  36. xCas9 3.6

    Plasmid
    #108384
    Purpose
    Mammalian expression vector for xCas9 3.6
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9 3.6
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108384-plasmid-map-sequence-id-207176

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  43. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid
    #58466
    Purpose
    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    58466-plasmid-map-sequence-id-410899

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Showing: 3801 - 3850 of 177146 results