162,717 results

1. HA-HA-BI-AR

   Plasmid

   #171234

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-APOBEC1-YTHmut

   Plasmid

   #178950

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTHmut-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRAD1

   Plasmid

   #47697

   Purpose

   General cloning vector for use in E. coli or D. radiodurans

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   repU

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. dTomato-2xrGBD

   Plasmid

   #129625

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   dTomato-2xrGBD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMVdel

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3puro-mGFP

   Plasmid

   #225446

   Purpose

   Express mEGFP-fusion protein of interest

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-DAXX-His

   Plasmid

   #175782

   Purpose

   DAXX protein for bacteria expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-N1-EXO1b

   Plasmid

   #111621

   Purpose

   Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGEN-dPspCas13b-NES-mCherry

   Plasmid

   #233609

   Purpose

   Expression of catalytically-inactive PspCas13b in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGEN-dPspCas13b-4EHP-NES-mCherry

   Plasmid

   #233610

   Purpose

   Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-4EHP-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRL278

    Plasmid

    #70256

    Purpose

    sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

    Depositor

    Peter Wolk

    Article

    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

    Type

    Empty backbone

    Use

    Conditionally lethal

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBNde(min)

    Plasmid

    #15125

    Depositor

    Franco Pagani

    Article

    Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

    Insert

    alpha-globin EDB fibronectin minigene (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    alpha-globin

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

15. AAV-HAPLN1

    Plasmid

    #200777

    Purpose

    Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_GNAI2_G-alpha

    Plasmid

    #109791

    Purpose

    Protein expression and purification of GNAI2_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNAI2_G-alpha (GNAI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQmod3C-GG

    Plasmid

    #191350

    Purpose

    Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIB185

    Plasmid

    #90196

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBR_attB(bxb)_ccdB_lox_v2

    Plasmid

    #198040

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-AktAR2

    Plasmid

    #64932

    Purpose

    Enhanced FRET-based biosensor for monitoring Akt activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    AktAR2
    

    Use

    Tags

    Cerulean3 and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNDGG054

    Plasmid

    #231323

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLEX305_3xFLAG_STAT1

    Plasmid

    #220323

    Purpose

    Expresses full length human STAT1 with an N-terminal 3xFLAG tag

    Depositor

    Jonathan Brown

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    PGK

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CRISPR-StAR4GN (Lenti)

    Plasmid

    #222693

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag Rab12 wild type

    Plasmid

    #86787

    Purpose

    mammalian expression of Rab12

    Depositor

    Peter McPherson

    Article

    Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

    Insert

    Rab12 (RAB12 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEV-G1-Km

    Plasmid

    #46813

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176269

    Purpose

    Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCasMINI-V4-VPR-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HDM_RSV_Long_G_31AACTdel

    Plasmid

    #237350

    Purpose

    Expression plasmid for RSV Long G

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV Long G from Genbank Accesion AY911262.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    31 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HDM_RSV_Long_F

    Plasmid

    #237349

    Purpose

    Expression plasmid for RSV Long F

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV Long F from Genbank Accesion AY911262.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

40. MIB2-FLAG

    Plasmid

    #37117

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only