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164,223 results

Showing: 4551 - 4600 of 164223 results

  1. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119299-plasmid-map-sequence-id-231827

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  4. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

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  6. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

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  10. pGMC00018 (aka pRC0082)

    Plasmid
    #195320
    Purpose
    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195320-plasmid-map-sequence-id-387102

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  11. pUC19 PKC alpha

    Plasmid
    #8429
    Depositor
    Frederic Mushinski
    Insert
    Mouse Protein Kinase C alpha (Prkca Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 5, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. pAB076i6

    Plasmid
    #79200
    Purpose
    Directed Evolution of Protein-Protein Interactions
    Depositor
    David Liu
    Article
    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:
    Insert
    PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacZ-opt (no operator); Ppro1
    Available Since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    79200-plasmid-map-sequence-id-148321

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  18. pEMS1602

    Plasmid
    #29305
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Article
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Insert
    Ple131 (MKI67 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29305-plasmid-map-sequence-id-13720

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  26. NanoLuc-CIT Fusion Vector

    Plasmid
    #237191
    Purpose
    Express NanoLuc(R)-CIT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CIT (CIT Human)
    Use
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237191-plasmid-map-sequence-id-471876

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  27. TEAD4-HaloTag BiBRET Vector

    Plasmid
    #237004
    Purpose
    Express TEAD4-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237004-plasmid-map-sequence-id-471870

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  30. Halo-ATG16L1 HRD

    Plasmid
    #207537
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    207537-plasmid-map-sequence-id-470412

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  31. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid
    #231953
    Purpose
    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    tTA_IRES_tagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231953-plasmid-map-sequence-id-470256

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  32. pENTR-B/c

    Plasmid
    #227962
    Purpose
    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Mutation
    Promoter
    None
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227962-plasmid-map-sequence-id-454806

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  39. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv November 07, 2024
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  43. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid
    #228735
    Purpose
    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv November 07, 2024
    Insert
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228735-plasmid-map-sequence-id-453996

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  46. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid
    #228734
    Purpose
    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv November 07, 2024
    Insert
    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228734-plasmid-map-sequence-id-453992

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  49. MLNR-DuET

    Plasmid
    #213339
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MLNR (MLNR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213339-plasmid-map-sequence-id-430913

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Showing: 4551 - 4600 of 164223 results