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167,150 results

Showing: 4551 - 4600 of 167150 results

  2. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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  11. pCK01_PalkS_alkS

    Plasmid
    #166502
    Purpose
    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.
    Depositor
    Sven Panke
    Article
    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2
    Insert
    alkS
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    L401G
    Promoter
    PalkS
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166502-plasmid-map-sequence-id-333027

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  16. pFD116

    Plasmid
    #124769
    Purpose
    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    dcas9
    PpflB sgRNA BsaI
    Use
    CRISPR
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124769-plasmid-map-sequence-id-246181

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  19. pShuttle

    Plasmid
    #16402
    Purpose
    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    16402-plasmid-map-sequence-id-237978

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  24. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Promoter
    T5 promoter/lac operator element
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  32. TFORF2307

    Plasmid
    #142023
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ETV1 (ETV1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142023-plasmid-map-sequence-id-395935

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  34. pTrEno

    Plasmid
    #115474
    Purpose
    Eno-promoter driven expression vector for protein production in Trichoderma reesei
    Depositor
    Steve Decker
    Article
    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d
    Insert
    Cel7A
    Use
    Unspecified
    Mutation
    Introns have been removed
    Promoter
    Trichoderma reesei Enolase (Eno) promoter
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    115474-plasmid-map-sequence-id-225594

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  37. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  43. pLVX TRE 3G BFP

    Plasmid
    #128070
    Purpose
    Lentiviral expression of Tet inducible TagBFP
    Depositor
    Oskar Laur
    Insert
    TagBFP
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128070-plasmid-map-sequence-id-255190

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  47. LV-AR-KKKAAA

    Plasmid
    #171219
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-KKKAAA (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K630A-K632A-K633A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171219-plasmid-map-sequence-id-342535

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Showing: 4551 - 4600 of 167150 results