8,630 results
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AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVTagsExpressionMutationPromoterU6 and cTnTAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xERRE/ERE-luciferase
Plasmid#37852DepositorInsert3 copies of ERRE/ERE upstream of luciferase
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLNCX myr Akt1
Plasmid#17245DepositorInsertAkt1 (Akt1 Mouse)
UseRetroviralTagsMyrExpressionMammalianMutationConstitutively activePromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
BoxB in pcDNA3
Plasmid#29727DepositorInsert5BoxB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
tol2-mpeg1-mcherry
Plasmid#58935Purposelabels macrophages with mcherryDepositorInsertmpeg1-mcherry
UseTagsExpressionMutationPromotermpeg1Available sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT317_pET-PhiC31o-pA
Plasmid#52491Purposefor preparation of PhiC31o mRNADepositorInsertPhiC31o integrase
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta K85A pcDNA3
Plasmid#14755DepositorInsertGSK3 beta K85A (GSK3B Human)
UseTagsHAExpressionMammalianMutationK85A. Kinase dead mutant.PromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCRII_TOPO_hNEAT1
Plasmid#61518Purposehuman NEAT1_v1 short isoform 3.7kb insert in general cloning vectorDepositorInsertNEAT1 (NEAT1 Human)
UseGeneral cloning vectorTagsExpressionMutation*see comment belowPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-GC3AI
Plasmid#78910PurposeIndicator of caspase-3-like protease activityDepositorInsertGC3AI
UseLentiviralTagsHA and MycExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-O-GECO1
Plasmid#46025PurposeOrange intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertO-GECO1
UseTagsExpressionMammalianMutationR-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP
Plasmid#75452PurposePlasmid for constitutive expression of gfpDepositorInsertgfp
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
human MMP-2 promoter
Plasmid#53432Purposepromoter activity studyDepositorInsertMMP2 promoter (MMP2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMMP2Available sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
amajLime chromoprotein
Plasmid#117843PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coliDepositorInsertpromoter, RBS, amajLime
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CAPN2
Plasmid#60942PurposeExpresses calpain2 in mammalian cellsDepositorInsertCalpain 2 (CAPN2 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleInsertCAN1.y gRNA (CAN1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOTTC476 - pAAV c-fos eYFP
Plasmid#47907PurposeAn AAV vector that expresses eYFP under the c-fos promoter.DepositorInsertenhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMutationPromoterc-fosAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOX6
Plasmid#127451PurposeEncodes Tse2 toxin and amilCP chromoprotein. CAM-RDepositorInsertstse2 toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-TREX1(D18N)
Plasmid#27220DepositorInsertTREX1 (TREX1 Human)
UseTagsGFPExpressionMammalianMutationD18N sequence 52-GAC changed to AAC, resulting i…PromoterAvailable sinceJan. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
ACTN2-pEGFP
Plasmid#52669PurposeOverexpression of GFP-tagged α-actinin-2DepositorInsertactinin 2 (ACTN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk3a2
Plasmid#13759DepositorInsertJnk3a2 (MAPK10 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pX330-SpCas9-NG
Plasmid#117919PurposeExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.DepositorInsertSpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin
Plasmid#60613PurposeEncodes YFP and NLS tagged beta-actinDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100853PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Parkin
Plasmid#17613DepositorInsertParkin (PRKN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro human IRS-1
Plasmid#11025DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorInsertGADD34 (PPP1R15A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTarget_CAST
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPalmitoyl-mTurquoise2
Plasmid#36209PurposeIn vivo visualization of the membrane (can be used for colocalization studies)DepositorInsertp63(1-29) (ARHGEF25 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-mCherry
Plasmid#108122PurposeRecruitable fluorophoreDepositorInsertFKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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p2xFlag CMV2-YAP2
Plasmid#19045DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-WT
Plasmid#32697DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationPromoterEF promoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-pBAD
Plasmid#54553PurposeLocalization: Protein Expression Vector , Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTags6xHis and mTFP1ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpRY-P2A-GFP
Plasmid#159981PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY-P2A-GFP
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro DEST (670-1)
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorInsertPKM2 (PKM Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sh1368 knockdown c-myc
Plasmid#29435DepositorInsertc-Myc shRNA (MYC Human)
UseLentiviral and RNAiTagsGFP and WPREExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Sox10
Plasmid#45843DepositorInsertSox10 (Sox10 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only
