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We narrowed to 9,416 results for: Coli

Showing: 4651 - 4700 of 9416 results

  1. pAIP4_3H6Q

    Plasmid
    #234185
    Purpose
    Heterologous protein expression of macrocypin-1a in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3H6Q
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234185-plasmid-map-sequence-id-464150

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  2. pAIP4_4Q2S

    Plasmid
    #234177
    Purpose
    Heterologous protein expression of C20G4.08 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4Q2S
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234177-plasmid-map-sequence-id-464142

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  3. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

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  4. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  5. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  6. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    8xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  7. pJBL7042

    Plasmid
    #167213
    Purpose
    overexpression of MerR in E. coli for cell-free mercury sensing
    Depositor
    Julius Lucks
    Insert
    MerR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167213-plasmid-map-sequence-id-330998

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  8. pJBL7043

    Plasmid
    #167214
    Purpose
    overexpression of ArsR in E. coli for cell-free arsenic sensing
    Depositor
    Julius Lucks
    Insert
    ArsR EP3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167214-plasmid-map-sequence-id-331005

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  9. pJBL7047

    Plasmid
    #167217
    Purpose
    overexpression of CadR in E. coli for cell-free cadmium sensing
    Depositor
    Julius Lucks
    Insert
    CadR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167217-plasmid-map-sequence-id-331011

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  10. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

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  11. pOP5BP

    Plasmid
    #112608
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112608-plasmid-map-sequence-id-220237

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  12. pET28b(+)-TM0848

    Plasmid
    #67287
    Purpose
    Expresses TM0848 in E. coli
    Depositor
    SeWon Suh
    Insert
    GTP-binding protein YqeH
    Use
    Tags
    N tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

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  14. pExp-CHis

    Plasmid
    #112559
    Purpose
    Used for expression of protein in E. coli as C-terminual 8His fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112559-plasmid-map-sequence-id-220671

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  15. pET28a-CYP168A1

    Plasmid
    #239982
    Purpose
    Expresses C-terminal 6xHis-tagged CYP168A1 in E. coli
    Depositor
    Justin Miller
    Insert
    cytochrome P450 CYP168A1
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239982-plasmid-map-sequence-id-484571

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  16. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  17. pAIP4_2NT3

    Plasmid
    #234208
    Purpose
    Heterologous protein expression of response regulator homolog in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2NT3
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234208-plasmid-map-sequence-id-464173

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  21. pPing

    Plasmid
    #47100
    Purpose
    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.
    Depositor
    Wayne Parrott
    Article
    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d
    Inserts
    Ping cDNA
    mPing
    hygromycin resistance gene
    Use
    Plant expression
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    CaMV35s and StUbi3
    Available Since
    Sept. 16, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47100-plasmid-map-sequence-id-67166

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  24. pORTMAGE-3

    Plasmid
    #72678
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72678-plasmid-map-sequence-id-198909

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  25. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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  34. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  35. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

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  41. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

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  42. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-457485

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  43. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available Since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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  44. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  45. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Promoter
    Available Since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  46. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

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  48. pET30a-His6-LOXCATmut

    Plasmid
    #140085
    Purpose
    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140085-plasmid-map-sequence-id-311165

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  50. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107454-plasmid-map-sequence-id-207518

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Showing: 4651 - 4700 of 9416 results