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166,201 results

Showing: 3761 - 3800 of 166201 results

  2. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  4. TFORF0481

    Plasmid
    #142638
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HIF1A (HIF1A Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142638-plasmid-map-sequence-id-397747

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  8. pBP Omomer

    Plasmid
    #113169
    Purpose
    Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.
    Depositor
    Sergio Nasi
    Article
    Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113169-plasmid-map-sequence-id-217942

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  17. TFORF2904

    Plasmid
    #143388
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DACH1 (DACH1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143388-plasmid-map-sequence-id-400329

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  20. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans trpC promoter
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

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  21. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Available Since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  22. pAd-RFP

    Plasmid
    #12520
    Depositor
    Tong-Chuan He
    Insert
    RFP
    Expression
    Mammalian
    Available Since
    Jan. 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    12520-plasmid-map-sequence-id-7339

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  24. pDB-His-MBP-SARM1

    Plasmid
    #195762
    Purpose
    Purification of SARM1 from E.coli
    Depositor
    Hao Wu
    Insert
    SARM1 (SARM1 Human)
    Tags
    HIS-MBP-TEV
    Expression
    Bacterial
    Mutation
    truncation of first 19 residues
    Promoter
    T7
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195762-plasmid-map-sequence-id-384751

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  27. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50470
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50470-plasmid-map-sequence-id-75489

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  28. TK4-EGFP

    Plasmid
    #239047
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239047-plasmid-map-sequence-id-473421

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  30. TFORF3135

    Plasmid
    #144611
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NOTCH2 (NOTCH2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144611-plasmid-map-sequence-id-346766

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  31. mEmerald-LASP1-N-10

    Plasmid
    #54142
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    NM_006148 (LASP1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    A209V
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  35. pGD-p19

    Plasmid
    #196326
    Purpose
    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor
    Depositor
    Zhenghe Li
    Article
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tomato bushy stunt virus p19
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196326-plasmid-map-sequence-id-390023

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  36. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  39. mCherry-PLK1-N-16

    Plasmid
    #55119
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    PLK1 (PLK1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55119-plasmid-map-sequence-id-424730

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Showing: 3761 - 3800 of 166201 results