163,682 results

1. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-EGFP.Flag-EZH2

   Plasmid

   #220243

   Purpose

   Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-VSVP

   Plasmid

   #64088

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV phosphoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-TOMM20-N-10

   Plasmid

   #55146

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-ADRBK1

   Plasmid

   #23835

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ADRBK1 (GRK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. PS6K-mcherry-iATPsnFR1.1

   Plasmid

   #227238

   Purpose

   Inducible ATP sensor for E. coli

   Depositor

   Fuzhong Zhang

   Article

   Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

   Insert

   iATPsnFR1.1
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. gag/pol

   Plasmid

   #14887

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   gag / pol
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-NPC1L1_STOP

   Plasmid

   #161469

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1L1 (NPC1L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pERM2-nhpSer

   Plasmid

   #201922

   Purpose

   Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   SepRS(2)
   Sep-tRNA v2.0
   SerB
   EF-Sep
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   E412P, E414F, T417K, P495M, I496W, F529S and H67R…

   Promoter

   GlnS (constitutive), OXB20 (constitutive), lpp (c…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-Neo-ISG15

    Plasmid

    #80404

    Purpose

    overexpression of ISG15

    Depositor

    Isidoro Martinez

    Article

    Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

    Plasmid

    #136904

    Purpose

    Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    mScarletGo-p2a-Blas
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5F-TACE

    Plasmid

    #31713

    Purpose

    Mammalian expression of human TACE (ADAM17)

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TACE (ADAM17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-UbC-VP64-dCas9-VP64

    Plasmid

    #232179

    Purpose

    Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    VP64-dCas9-VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLRP3-mNeonGreen

    Plasmid

    #200942

    Purpose

    NLRP3 fused with mNeonGreen to indicate its localization change

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ApoE3

    Plasmid

    #197147

    Purpose

    Expresses ApoE3 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBZS-M.CviPII (NDel15)

    Plasmid

    #198356

    Purpose

    Express M.CviPII (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGWB402SC

    Plasmid

    #224552

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep-HA

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

31. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL3Luc-5XNF-kappaB

    Plasmid

    #185695

    Purpose

    Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

    Depositor

    Esther López-Bayghen

    Article

    Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

    Insert

    NF-kappaB (NFKB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    NF-kappaB binding site

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available Since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge