159,371 results

1. pHR_CMV_MeltNLS/NES-40-mCherry

   Plasmid

   #234066

   Purpose

   Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltNLS/NES-40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reporter-1

   Plasmid

   #180218

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221_SLC1A2

   Plasmid

   #131901

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A2 (SLC1A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pK7WG2-mitoTAL2-orf125

   Plasmid

   #158737

   Purpose

   Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   mitoTALEN left and right to target orf125 (orf138)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA AXL

   Plasmid

   #105932

   Purpose

   Mammalian expression of myc-His tagged AXL

   Depositor

   Rosa Marina Melillo

   Article

   Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

   Insert

   AXL (AXL Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Promoter

   tet-inducible CMV

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LZRS-HOTAIR

    Plasmid

    #26110

    Depositor

    Howard Chang

    Article

    Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

    Insert

    HOTAIR (HOTAIR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-sCAG-mBaoJin-oPRE

    Plasmid

    #218684

    Purpose

    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Short variant of the CAG promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPO4

    Plasmid

    #209978

    Purpose

    Contains Level 0 Part: replication origin (pWV01) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (pWV01)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221_SLC38A3

    Plasmid

    #132142

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A3 (SLC38A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC7A10

    Plasmid

    #132232

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A10 (SLC7A10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pDONR221_SLC38A8

    Plasmid

    #132082

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A8 (SLC38A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPath-Violacein-native

    Plasmid

    #200001

    Purpose

    Violacein pathway. Native promoter and CDS sequence

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SPDK3959

    Plasmid

    #199246

    Purpose

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

    Insert

    Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    Promoter

    PEBV

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-PEAR-mCherry

    Plasmid

    #228892

    Purpose

    All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Insert

    EGFP-IRES-mScarlet
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LM627

    Plasmid

    #191551

    Purpose

    LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    SNAC-tag, HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pG2K

    Plasmid

    #71742

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pZP06C

    Plasmid

    #232322

    Purpose

    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

    Depositor

    Chenggang Wu

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsJ

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCSC-NGN2-IRES-ISL1-T2A-LHX3

    Plasmid

    #221814

    Purpose

    Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

    Depositor

    Baojin Ding

    Article

    Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

    Inserts

    NEUROG2 (NEUROG2 Human)
    ISL1 (ISL1 Human)
    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-tdTomato (codon diversified)

    Plasmid

    #59462

    Purpose

    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    codon diversified

    Promoter

    CAG

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge