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173,478 results

Showing: 10451 - 10500 of 173478 results

  2. mPlum-VE-Cadherin-N-10

    Plasmid
    #56005
    Purpose
    Localization: Tight Junctions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    VE-Cadherin (CDH5 Human)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    I503T
    Promoter
    CMV
    Available Since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    56005-plasmid-map-sequence-id-474989

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  5. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  6. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  7. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  11. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV Retrograde)

    Viral Prep
    #26975-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CamKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. CMV-LgBiT-AR Expression Vector

    Plasmid
    #237117
    Purpose
    Express LgBiT-AR Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    AR (AR Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237117-plasmid-map-sequence-id-485900

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  14. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

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  15. TFORF1338

    Plasmid
    #142787
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZBTB7C (ZBTB7C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142787-plasmid-map-sequence-id-462899

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  20. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49436-plasmid-map-sequence-id-251833

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  22. KE-009

    Plasmid
    #133441
    Purpose
    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4H (EIF4H Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133441-plasmid-map-sequence-id-267720

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  31. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111026-plasmid-map-sequence-id-214044

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  39. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  40. pCAGGS-ROSAr

    Plasmid
    #54324
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Expression
    Mammalian
    Promoter
    CAGG
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54324-plasmid-map-sequence-id-124429

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  50. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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Showing: 10451 - 10500 of 173478 results