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179,539 results

Showing: 10451 - 10500 of 179539 results

  2. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161928-plasmid-map-sequence-id-325287

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  4. pC1-mitoRexYFP

    Plasmid
    #60246
    Purpose
    Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.
    Depositor
    Vsevolod Belousov
    Publication
    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mitoRexYFP
    Tags
    Mitochondrial targeting signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60246-plasmid-map-sequence-id-317300

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  5. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

    Viral prep
    #137163-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre-dependent)
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-Ef1a-Con/Foff 2.0-GCaMP6M (AAV8)

    Viral prep
    #137120-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-GCaMP6M (#137120). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-GCaMP6M plasmid DNA. EF1a-driven expression of GCaMP6m in the presence of Cre recombinase (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only

  10. pHes7-Achilles-Hes7

    Plasmid
    #153528
    Purpose
    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.
    Depositor
    Ryoichiro Kageyama
    Publication
    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hes7 (Hes7 Mouse)
    Tags
    Achilles
    Expression
    Mammalian
    Promoter
    mouse Hes7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153528-plasmid-map-sequence-id-305297

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  13. pcDNA3.1-Beta3

    Plasmid
    #140988
    Purpose
    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBeta3 (GNB3 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    140988-plasmid-map-sequence-id-301591

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  14. SPACE (pRZ1813)

    Plasmid
    #140242
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE
    Expression
    Mammalian
    Mutation
    V82G in TadA*, D10A in Cas9, R187W in pmCDA1
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140242-plasmid-map-sequence-id-275730

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  15. P362-FBP_12

    Plasmid
    #139703
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Publication
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139703-plasmid-map-sequence-id-296521

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  16. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Publication
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    122239-plasmid-map-sequence-id-292012

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  20. UAS-mCherry

    Plasmid
    #135457
    Purpose
    Mammalian mCherry reporter to read out FLARE and SPARK activation
    Depositor
    Alice Ting
    Publication
    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    UAS
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135457-plasmid-map-sequence-id-267451

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  29. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Publication
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    117986-plasmid-map-sequence-id-235371

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  32. TNFR1-YFP

    Plasmid
    #111209
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TNFRSF1A (TNFRSF1A Human)
    Tags
    EYFP
    Expression
    Mammalian
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111209-plasmid-map-sequence-id-212987

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  35. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    111431-plasmid-map-sequence-id-292191

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  44. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    90518-plasmid-map-sequence-id-288271

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  48. mEGFP-PI35P2

    Plasmid
    #92419
    Purpose
    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.
    Depositor
    Geert van den Bogaart
    Publication
    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCOLN1 (Mcoln1 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Synthetic gene, codon optimized for human.
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92419-plasmid-map-sequence-id-182509

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Showing: 10451 - 10500 of 179539 results