20,807 results
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Plasmid#97043PurposeExpresses RUNX1 in mammalian cellsDepositorInsertRunt Related Transcription Factor 1 (RUNX1 Human)
UseLentiviralExpressionMammalianPromoterTet onAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dronpa3-C1
Plasmid#54655PurposeLocalization: C1 Cloning Vector, Excitation: 503, Emission: 518DepositorTypeEmpty backboneTagsDronpa3ExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC-sleeping beauty
Plasmid#65487PurposeHSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.DepositorInsertsleeping beauty transposase
ExpressionInsectPromoterHSPAvailable SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
TagsHexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28a SnoopTag-MBP
Plasmid#72323PurposeBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.DepositorInsertpET28a SnoopTag-MBP
TagsN-terminal His6 tag. MBP is fused at the C-termin…ExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEP179_pX330K
Plasmid#137882PurposeEntry vector for multi-gRNA cloningDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX
Plasmid#167583PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cellsDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
ExpressionMammalianPromoterCMVAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo beta catenin S33Y
Plasmid#16519DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
Plasmid#84243PurposeExpresses ABA-inducible VPR-Sa dCas9DepositorInsertsABI-tagBFP-Sa dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKC epsilon DN
Plasmid#21243DepositorAvailable SinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-Cre
Plasmid#50935Purposeretroviral expression of Cre recombinaseDepositorInsertCre recombinase
UseCre/Lox and RetroviralTagsNLSExpressionMammalianAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-somBiPOLES-mCerulean
Plasmid#154945PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
NPY-GFP
Plasmid#74629Purposehuman neuropeptide Y fused to GFPDepositorAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA1 TKIT
Plasmid#169441PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAPK1_WT
Plasmid#82145PurposeGateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FU - dio YFP - W
Plasmid#73858PurposeFor expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.DepositorInserteYFP
UseLentiviralExpressionMammalianAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP703
Plasmid#79998PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703DepositorAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
COX8-SuperNova
Plasmid#131856PurposeExpresses SuperNova fused to mitochrondrial protein COX8ADepositorInsertSuperNova
TagsCox8A and Myc tagExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
MPZ(P0)promoter pGL3-TATA
Plasmid#21259DepositorAvailable SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
Plasmid#58442PurposeCassette to replace yeast promoters with an inducible promoter containing 4 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
ExpressionYeastPromoterPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (noNLS) nog
Plasmid#119554PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1P-iCAG.copGFP
Plasmid#66577PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENsDepositorInsertcopGFP
ExpressionMammalianPromoterCAGAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2
Plasmid#109020Purposestable lentiviral expression of cDNADepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP670nano3
Plasmid#184670PurposeHistone 2B labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertH2B-miRFP670nano3
TagsmiRFP670nano3ExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-DEVD-NanoLuc
Plasmid#98287Purposecaspase-3 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
TagsHis-tagExpressionMammalianPromoterCMVAvailable SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_WT
Plasmid#81923PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-TEV protease
Plasmid#165509PurposeUsed for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/GDepositorInsertTEV protease
UseSynthetic BiologyAvailable SinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-S5E2-GCaMP6f
Plasmid#135632PurposeAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertGCaMP6f
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-pre-eGRASP(p32)
Plasmid#111598PurposeAn AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertgreen pre-eGRASP(p32)
UseAAVPromoterCaMKIIaAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM33
Plasmid#65398PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP_a_tubulin_IRES_puro2b
Plasmid#21042DepositorAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRho-DsRed
Plasmid#11156DepositorAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mCherry-EGFP-Tet-On
Plasmid#109017PurposeTet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)DepositorInsertPMP34 (SLC25A17 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianPromoterCMV promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL wt-pBabe-puro
Plasmid#19234DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwtAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
Tagshuman H2BExpressionInsectPromoter10xUASAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEmCherry2
Plasmid#118413PurposeRed fluorescent reporter for assessing CRISPR gRNA activityDepositorInsertmCherry
UseCRISPRExpressionMammalianAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26
Plasmid#86233PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
1319 pcDNA3 flag FKHRL1 AAA
Plasmid#10709DepositorInsertFKHRL1 AAA (FOXO3 Human)
TagsflagExpressionMammalianMutationAAA mutant (see map) - T32A, S253A, S315AAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-SIN6.1 CGFPW
Plasmid#44171DepositorInsertenhanced green fluorescent protein
UseLentiviralMutationchromophore mutationsPromoterCMVAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SETDB1_WT
Plasmid#82291PurposeGateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYOD1-3xHA (#730)
Plasmid#78328PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tagDepositorAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_H2A_C1
Plasmid#85051PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Farnesyl-5
Plasmid#58092PurposeLocalization: Membrane, Excitation: 554, Emission: 581DepositorInsertFarnesyl (HRAS Human)
TagstdTomatoExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceDec. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
tetO-MEF2C
Plasmid#46031DepositorAvailable SinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
APJ-Tango
Plasmid#66224PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only