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179,463 results

Showing: 1701 - 1750 of 179463 results

  1. pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)

    Viral prep
    #26973-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    EYFP
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only

  2. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid
    #108570
    Purpose
    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi
    Insert
    dSpyCas9
    Use
    CRISPR and Lentiviral
    Tags
    APEX2, Destabilization domains, Flag, and mCherry…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108570-plasmid-map-sequence-id-328916

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  13. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Expression
    Mammalian
    Available since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    1732-plasmid-map-sequence-id-326991

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  15. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    62202-plasmid-map-sequence-id-288285

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  17. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has service
    AAV1 and AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    51085-plasmid-map-sequence-id-148706

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  25. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

    Viral prep
    #87306-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Publication
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only

  26. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    103865-plasmid-map-sequence-id-243540

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  28. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    89180-plasmid-map-sequence-id-176308

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  36. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV9)

    Viral prep
    #26966-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1701 - 1750 of 179463 results