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175,655 results

Showing: 2901 - 2950 of 175655 results

  4. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  17. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available Since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  21. pCAG-B19L

    Plasmid
    #59922
    Purpose
    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus RNA dependent RNA polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    59922-plasmid-map-sequence-id-94980

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  28. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

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  31. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid
    #84563
    Purpose
    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    Use
    Lentiviral
    Tags
    myc tag (9x)
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84563-plasmid-map-sequence-id-176790

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  32. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105530-plasmid-map-sequence-id-454420

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  42. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  49. pEVOL_PylRS(WT)-tRNA

    Plasmid
    #223512
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    223512-plasmid-map-sequence-id-458970

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Showing: 2901 - 2950 of 175655 results