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179,463 results

Showing: 3101 - 3150 of 179463 results

  1. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    20 Articles
    72266-plasmid-map-sequence-id-447561

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  3. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Publication
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    63891-plasmid-map-sequence-id-450336

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  18. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    139462-plasmid-map-sequence-id-308474

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  22. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral prep
    #100798-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Publication
    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. AAV-EF1a-BbChT (AAV9)

    Viral prep
    #45186-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Dawen Cai, Joshua Sanes
    Publication
    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi
    Promoter
    EF1a
    Tags
    mCherry, mTFP, or none
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  26. AAV-EF1a-BbTagBY (AAV9)

    Viral prep
    #45185-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Dawen Cai, Joshua Sanes
    Publication
    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi
    Promoter
    EF1a
    Tags
    TagBFP, EYFP, or none.
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV1)

    Viral prep
    #51085-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  36. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    45945-plasmid-map-sequence-id-116251

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  48. FCK-Halo-GFP

    Plasmid
    #14750
    Purpose
    Lentiviral expression of Halo-GFP for neuronal inhibition
    Depositor
    Edward Boyden
    Publication
    Han et al PLoS One. 2007 Mar 21;2(3):e299.
    Insert
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N. pharaonis halorhodopsin is mammalian codon-opt…
    Promoter
    CaMKIIa
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    14750-plasmid-map-sequence-id-192940

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Showing: 3101 - 3150 of 179463 results