179,463 results

Showing: 3351 - 3400 of 179463 results

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMSCV-IRES-Blue FP

Plasmid

#52115

Purpose

a bicistronic IRES-Blue FP containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-Blue FP

Expression

Mammalian

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Publication

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

hSyn1

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Human Lentiviral sgRNA Library - Nuclear

Pooled library

#51047

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.

Depositor

Eric Lander, David Sabatini

Publication

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Publication

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-STAT6-IRES-Neo

Plasmid

#35482

Depositor

Axel Nohturfft

Insert

STAT6 (STAT6 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Publication

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCDNA 3 5' cMyc SMC1 wt

Plasmid

#32363

Depositor

Michael Kastan

Publication

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C1-ER beta

Plasmid

#28237

Depositor

Michael Mancini

Insert

ER beta (ESR2 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-hSyn-hChR2(H134R)-mCherry

Plasmid

#26976

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

Depositor

Karl Deisseroth

Has service

AAV5, AAV8, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Publication

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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ChR2-2A-Halo

Plasmid

#22047

Purpose

Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

Depositor

Edward Boyden

Publication

Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

Insert

ChR2-GFP-2A-Halo-YFP

Tags

GFP and YFP

Expression

Mammalian

Mutation

Note that there is a S147R mut in YFP. This mutat…

Promoter

CMV

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

Plasmid

#20299

Purpose

Cre-activated AAV expression of mCherry

Depositor

Karl Deisseroth

Has service

AAV9

Insert

mCherry

Use

AAV

Expression

Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDONR207 SARS-CoV-2 M

Plasmid

#141274

Purpose

Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

M (M SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Publication

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDEST-CMV-C-EGFP

Plasmid

#122844

Purpose

C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Publication

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Promoter

MSCV

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Flag-miniTurbo

Plasmid

#124647

Purpose

Expresses Flag-miniTurbo fusion for proximity labeling

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.hSynap.iGABASnFR

Plasmid

#112159

Purpose

GABA Sensor

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has service

AAV1

Insert

iGABASnFR

Use

AAV

Promoter

hSynapsin

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pN1-GCaMP6m-XC

Plasmid

#111543

Purpose

Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

Depositor

Xiaodong Liu

Publication

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XC

Expression

Mammalian

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV1)

Viral prep

#26971-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

CaMKII

Tags

EYFP

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-hYTHDC1

Plasmid

#85167

Purpose

Expression of FLAG-HA-tagged human YTHDC1

Depositor

Samie Jaffrey

Publication

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDC1 (YTHDC1 Human)

Tags

Flag and Ha

Expression

Mammalian

Promoter

CMV

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCytERM_mScarlet_N1

Plasmid

#85066

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCW57-MCS1-P2A-MCS2 (RFP)

Plasmid

#80923

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Jie Chen, Taekjip Ha

Publication

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Publication

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDisplay-pHuji

Plasmid

#61556

Purpose

Red fluorescent protein based pH indicator in pDisplay vector

Depositor

Robert Campbell

Publication

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

pHuji

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA4TO-24xGCN4_v4-sfGFP

Plasmid

#61058

Purpose

A direct fusion of sfGFP to the 24xGCN4_v4 peptide array

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v4 peptide array and sfGFP

Expression

Mammalian

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Expression

Bacterial

Promoter

T7/Sp6

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

17 Articles

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pMSCV-IRES-DsRed FP (pMIDsRed II)

Plasmid

#52110

Purpose

a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Publication

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-DsRed

Expression

Mammalian

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGFP FAK

Plasmid

#50515

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Publication

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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LPC-flag-REST-WT

Plasmid

#41903

Depositor

Stephen Elledge

Publication

Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

Insert

RE1-silencing transcription factor (REST Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
shYAP1 # 2

Plasmid

#42541

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

Enlarge
pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Publication

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
Pseudojanin (PJ)

Plasmid

#37999

Depositor

Robin Irvine

Publication

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

Enlarge
pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pLSSmKate2-N1

Plasmid

#31867

Depositor

Vladislav Verkhusha

Publication

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Publication

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

Enlarge
pDONR223_Luciferase

Plasmid

#25894

Purpose

Gateway entry vector containing Luciferase.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

Luciferase

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pBabe puro Snail

Plasmid

#23347

Depositor

Bob Weinberg

Publication

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

Snail (SNAI1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pEF-ENTR A (696-6)

Plasmid

#17427

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame A

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pAAV-double floxed-eNpHR-EYFP-WPRE-pA

Plasmid

#20949

Purpose

Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

Depositor

Karl Deisseroth

Has service

AAV9

Insert

halorhodopsin

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

EF1alpha

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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